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GeneCorner plasmid details

Last data update: 28 October 2020 04:17 CET

Plasmid name: p416-SHS-TI (LMBP 11195)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p11195.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Candida albicans SAT1 gene disruption cassette
Saccharomyces cerevisiae 2µ plasmid site-specific recombinase Flp cDNA (FLP1); with Candida albicans TUB2 intron
Promoter: Candida albicans elongation factor 1-alpha 2 promoter (TEF2)
Candida albicans alpha-glucosidase promoter (MAL2)
Escherichia coli lac operon promoter
Phage T7 gene 10 promoter (T7g10)
Candida albicans actin promoter (ACT1)
Ribosome
binding site:
-
Terminator: Candida albicans ATP-dependent 6-phosphofructokinase subunit beta terminator (PFK2)
Candida albicans actin terminator (ACT1)
Selection marker: Ampicillin (amp)
Hygromycin (hyg)
Saccharomyces cerevisiae URA3; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4
Host range: Candida albicans; ura3(-)
Saccharomyces cerevisiae; ura3(-)
Escherichia coli
Parental clone: p416-SHS
Further information: The plasmid was constructed by cloning the Candida albicans TUB2 intron into the FLP coding sequence of p416-SHS.
The plasmid is intended for gene deletion in Candida albicans.
The nucleotide sequence of the plasmid corresponds with the EMBL Nucleotide Sequence Database accession number MF048870.1.
EMBL Accession number: MF048870.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 26/03/2019
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by Dr K. Ganesan(1). It was constructed by Dr S. Bijlani(1).
(1) CSIR Institute of Microbial Technology, Chandigarh, India
Plasmid reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

p416-SHS-TI (LMBP 11195) is available at BCCM/GeneCorner. The plasmid was deposited by Dr K. Ganesan.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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