Last data update: 24 January 2024 16:39 CET
Plasmid name: p416MET25 (LMBP 4018)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p4018.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | Saccharomyces cerevisiae O-acetyl homoserine sulfhydrylase promoter (MET25) Escherichia coli lac operon promoter Phage T3 promoter Phage T7 gene 10 promoter (T7g10); synthetic sequence |
Ribosome binding site: |
- |
Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic |
Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4 Phage f1 origin |
Copy number: | Low copy number |
Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
Parental clone: | pRS416 |
Further information: | This plasmid is a yeast expression shuttle vector. The expression from the S. cerevisiae O-acetyl homoserine sulfhydrylase promoter (MET25) is repressed when cells are grown in the presence of methionine. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. Other name of the plasmid is p416 MET25. |
EMBL Accession number: | - |
Latest sequence update: | 17/01/2000 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr R. Müller(1). (1) Philipps University of Marburg, School of Medicine, Institute of Molecular Biology and Tumor Research (IMT) - Center for Tumor Biology and Immunology (ZTI), Marburg, Germany |
Plasmid reference: | Mumberg et al., Nucleic Acids Res. 22 (1994), 5767-5768 [PMID: 7838736] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12xB HB101 |
Host reference: | Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022] |
Related host reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | ATCC 87326 |
Refer in your Materials and Methods: |
p416MET25 (LMBP 4018) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Müller and was published in Mumberg et al., 1994. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.