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GeneCorner plasmid details

Last data update: 22 October 2020 04:25 CEST

Plasmid name: p425GALLmBAX (LMBP 4576)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4576.gb (View with Genome Compiler)
p4576.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse Bcl2-associated X cDNA (Bax, BAX); α variant
Promoter: Escherichia coli lac operon promoter
Phage T3 promoter
Phage T7 gene 10 promoter (T7g10); synthetic sequence
Saccharomyces cerevisiae galactokinase promoter (GAL1); with deletion of one of the three UAS elements (GALL)
Ribosome
binding site:
-
Terminator: Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Selection marker: Ampicillin (amp)
LEU2; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB)
Host range: Escherichia coli
Saccharomyces cerevisiae; leu2(-)
Parental clone: p425GALL; pSCGAL1mBAX-L
Further information: The plasmid was constructed by substituting the HindIII-SpeI fragment from p425GALL by the HindIII-XbaI fragment from pSCGAL1mBAX-L, containing the α variant of the mouse Bcl2-associated X cDNA (mBAX).
p425GALLmBAX is a high-copy number shuttle vector for expression of the mouse Bax-α protein.
The GALL promoter is a deletion variant of the S. cerevisiae galactokinase promoter (GAL1), which lacks one of the three UAS elements required for full induction of the promoter by galactose. The GALL promoter is tightly repressed by glucose and is induced at a moderate level by galactose.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of the mBAX cDNA corresponds with the EMBL Nucleotide Sequence Database accession number L22472.1.
Other name of the plasmid is p425GALLBAX.
EMBL Accession number: L22472.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 07/03/2003
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BanII, BglI/HindII, HindIII/XbaI and XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Contreras(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Mumberg et al., Nucleic Acids Res. 22 (1994), 5767-5768 [PMID: 7838736]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

p425GALLmBAX (LMBP 4576) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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