Last data update: 26 November 2020 04:30 CET
Plasmid name: p425MET25 (LMBP 4017)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Promoter:||Saccharomyces cerevisiae O-acetyl homoserine sulfhydrylase promoter (MET25)
Escherichia coli lac operon promoter
Phage T3 promoter
Phage T7 gene 10 promoter (T7g10); synthetic sequence
|Terminator:||Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)|
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin
Saccharomyces cerevisiae 2 micron plasmid origin (2μ), incl. region conferring stability (STB)
Phage f1 origin
|Copy number:||High copy number|
|Host range:||Escherichia coli
Saccharomyces cerevisiae; leu2(-)
|Further information:||This plasmid is a yeast expression shuttle vector.
The expression from the S. cerevisiae O-acetyl homoserine sulfhydrylase promoter (MET25) is repressed when cells are grown in the presence of methionine.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
Other name of the plasmid is p425 MET25.
|EMBL Accession number:||-|
|Latest sequence update:||17/01/2000|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr R. Müller(1).
(1) Philipps University of Marburg, School of Medicine, Institute of Molecular Biology and Tumor Research (IMT) - Center for Tumor Biology and Immunology (ZTI), Marburg, Germany
|Plasmid reference:||Mumberg et al., Nucleic Acids Res. 22 (1994), 5767-5768 [PMID: 7838736]
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12xB HB101|
|Host reference:||Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
|Related host reference:||Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||ATCC 87323|
Refer in your Materials and Methods:
|p425MET25 (LMBP 4017) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Müller and was published in Mumberg et al., 1994.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.