Last data update: 12 May 2021 19:50 CEST
Plasmid name: pA-HYG OSCAR (LMBP 8056)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Promoter:||Aspergillus nidulans tryptophan C promoter (trpC)
Escherichia coli lac operon promoter
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
|Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)|
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli
|Parental clone:||pBluescriptIIKS+; pDONR-P4-P1R; pDONR-A-Hyg|
|Further information:||The plasmid was created as follows: 1) the attP1r sites was isolated as a NotI/SalI fragment from pDONR-P4-P1R and cloned into the NotI/SalI opened pBluescriptKS+ vector, generating pBS-P1R, 2) the attP4 sites was isolated as a ApaI/XmnI fragment from pDONR-P4-P1R and cloned into the SmaI-opened pBluescriptKS+ vector, generating pBS-P4, 3) the hygromycin resistance cassette was isolated as a HpaI fragment from pDONR-A-Hyg and cloned into the SmaI-opened pBluescriptKS+, generating pBS-A-Hyg 4) the hygromycin resistance cassette was isolated as a EcoRI/NotI fragment from pBS-A-Hyg and cloned into the EcoRI/NotI-opened pBS-P1R, generating pBS-P1R-A-Hyg and 5) the attP4 site was isolated as an EcoRI/SacI fragment from pBS-P4, the fragment was blunted and cloned into the NotI-opened and blunted pBS-P1R-A-Hyg, generating pA-HYG OSCAR.
Name used in Paz et al (2011) is pA-Hyg-OSCAR.
The nucleotide sequence of the pOSCAR vector corresponds with the EMBL Nucleotide Sequence Database accession number HM623915.1.
|EMBL Accession number:||HM623915.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||14/01/2013|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: KpnI, PvuI and SacII/SalI.|
|History of deposit:||This plasmid was deposited by Prof. Dr K. McCluskey(1).
(1) FGSC, University of Missouri, Kansas City, USA
|Plasmid reference:||Paz et al., Fungal Genet. Biol. 48 (2011), 677-684 [PMID: 21362493] [DOI: 10.1016/j.fgb.2011.02.003]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pA-HYG OSCAR (LMBP 8056) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. McCluskey and was published in Paz et al., 2011.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.