Last data update: 24 January 2024 16:39 CET
Plasmid name: pA-HYG OSCAR (LMBP 8056)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p8056.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Aspergillus nidulans tryptophan C promoter (trpC) Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) Phage T3 promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Ascomycete fungi |
| Parental clone: | pBluescriptIIKS+; pDONR-P4-P1R; pDONR-A-Hyg |
| Further information: | The plasmid was created as follows: 1) the attP1r sites was isolated as a NotI/SalI fragment from pDONR-P4-P1R and cloned into the NotI/SalI opened pBluescriptKS+ vector, generating pBS-P1R, 2) the attP4 sites was isolated as a ApaI/XmnI fragment from pDONR-P4-P1R and cloned into the SmaI-opened pBluescriptKS+ vector, generating pBS-P4, 3) the hygromycin resistance cassette was isolated as a HpaI fragment from pDONR-A-Hyg and cloned into the SmaI-opened pBluescriptKS+, generating pBS-A-Hyg 4) the hygromycin resistance cassette was isolated as a EcoRI/NotI fragment from pBS-A-Hyg and cloned into the EcoRI/NotI-opened pBS-P1R, generating pBS-P1R-A-Hyg and 5) the attP4 site was isolated as an EcoRI/SacI fragment from pBS-P4, the fragment was blunted and cloned into the NotI-opened and blunted pBS-P1R-A-Hyg, generating pA-HYG OSCAR. Name used in Paz et al (2011) is pA-Hyg-OSCAR. The nucleotide sequence of the pOSCAR vector corresponds with the EMBL Nucleotide Sequence Database accession number HM623915.1. |
| EMBL Accession number: | HM623915.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 14/01/2013 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: KpnI, PvuI and SacII/SalI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr K. McCluskey(1). (1) FGSC, University of Missouri, Kansas City, USA |
| Plasmid reference: | Paz et al., Fungal Genet. Biol. 48 (2011), 677-684 [PMID: 21362493] [DOI: 10.1016/j.fgb.2011.02.003] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pA-HYG OSCAR (LMBP 8056) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. McCluskey and was published in Paz et al., 2011. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.