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GeneCorner plasmid details

Last data update: 20 October 2020 04:14 CEST

Plasmid name: pA-HYG OSCAR (LMBP 8056)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p8056.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Aspergillus nidulans tryptophan C promoter (trpC)
Escherichia coli lac operon promoter
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: -
Selection marker: Ampicillin (amp)
Hygromycin (hyg)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Ascomycete fungi
Parental clone: pBluescriptIIKS+; pDONR-P4-P1R; pDONR-A-Hyg
Further information: The plasmid was created as follows: 1) the attP1r sites was isolated as a NotI/SalI fragment from pDONR-P4-P1R and cloned into the NotI/SalI opened pBluescriptKS+ vector, generating pBS-P1R, 2) the attP4 sites was isolated as a ApaI/XmnI fragment from pDONR-P4-P1R and cloned into the SmaI-opened pBluescriptKS+ vector, generating pBS-P4, 3) the hygromycin resistance cassette was isolated as a HpaI fragment from pDONR-A-Hyg and cloned into the SmaI-opened pBluescriptKS+, generating pBS-A-Hyg 4) the hygromycin resistance cassette was isolated as a EcoRI/NotI fragment from pBS-A-Hyg and cloned into the EcoRI/NotI-opened pBS-P1R, generating pBS-P1R-A-Hyg and 5) the attP4 site was isolated as an EcoRI/SacI fragment from pBS-P4, the fragment was blunted and cloned into the NotI-opened and blunted pBS-P1R-A-Hyg, generating pA-HYG OSCAR.
Name used in Paz et al (2011) is pA-Hyg-OSCAR.
The nucleotide sequence of the pOSCAR vector corresponds with the EMBL Nucleotide Sequence Database accession number HM623915.1.
EMBL Accession number: HM623915.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 14/01/2013
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: KpnI, PvuI and SacII/SalI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr K. McCluskey(1).
(1) FGSC, University of Missouri, Kansas City, USA
Plasmid reference: Paz et al., Fungal Genet. Biol. 48 (2011), 677-684 [PMID: 21362493] [DOI: 10.1016/j.fgb.2011.02.003]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pA-HYG OSCAR (LMBP 8056) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. McCluskey and was published in Paz et al., 2011.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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