Last data update: 11 June 2021 14:12 CEST
Plasmid name: pA13 (LMBP 9595)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9595.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli lac Z gene (lacZ); 5' fragment with modified 5' end |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Erythromycin (ery) |
| Replicon: | Lactobacillus plantarum A112 plasmid pA1 origin, incl. repA and repB |
| Copy number: | High copy number |
| Host range: | Escherichia coli Lactobacillus sp. Lactococcus sp. |
| Parental clone: | pIL253; pA1; pUC19 |
| Further information: | The plasmid was constructed by combining the origin of replication from the L. plantarum A112 pA1 plasmid, the lacZ gene with multiple cloning site from pUC19 and the erythromycin resistance gene from pIL253. This plasmid is a high copy shuttle-cloning vector between Escherichia coli, Lactobacillus and Lactococcus strains. The plasmid is suitable for cloning large fragments (more than 14 kb). The nucleotide sequence of the pA13 vector corresponds with the EMBL Nucleotide Sequence Database accession number FM212242.1. |
| EMBL Accession number: | FM212242.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 03/08/2015 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr M. Kojic(1). (1) Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia |
| Plasmid reference: | Kojic et al., Int. J. Food Microbiol. 140 (2010), 117-24 [PMID: 20439125] [DOI: 10.1016/j.ijfoodmicro.2010.04.010] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + erythromycin (150 µg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pA13 (LMBP 9595) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Kojic and was published in Kojic et al., 2010. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.