Last data update: 24 January 2024 16:39 CET
Plasmid name: pAB-c-Rel (LMBP 5676)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p5676.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human v-rel reticuloendotheliosis viral oncogene homolog (REL) cDNA |
Promoter: | Phage T7 gene 10 promoter (T7g10) Phage T3 promoter Escherichia coli lac operon promoter |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Further information: | The nucleotide sequence of the hREL cds corresponds with the EMBL Nucleotide Sequence Database accession number DQ314888.1. |
EMBL Accession number: | DQ314888.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 26/02/2009 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr K. De Bosscher(1) and Prof. Dr Guy Haegeman(1). It was constructed by Dr S. Plaisance(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pAB-c-Rel (LMBP 5676) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Bosscher and Prof. Dr Guy Haegeman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.