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GeneCorner plasmid details

Last data update: 29 October 2020 04:15 CET

Plasmid name: pAB-c-Rel (LMBP 5676)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p5676.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human v-rel reticuloendotheliosis viral oncogene homolog (REL) cDNA
Promoter: Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Further information: The nucleotide sequence of the hREL cds corresponds with the EMBL Nucleotide Sequence Database accession number DQ314888.1.
EMBL Accession number: DQ314888.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 26/02/2009
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. De Bosscher(1) and Prof. Dr Guy Haegeman(1). It was constructed by Dr S. Plaisance(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pAB-c-Rel (LMBP 5676) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Bosscher and Prof. Dr Guy Haegeman.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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