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GeneCorner plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pAB-jun (LMBP 5040)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p5040.gb (View with Genome Compiler)
p5040.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd)
Mouse c-jun oncogene cDNA (mcjun)
Promoter: Rous sarcoma virus long terminal repeat (RSV-LTR)
Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pABgal
Further information: The plasmid was constructed by inserting the mouse c-jun oncogene (mcjun) cDNA into the PvuII opened pAB-gal vector. As a result, the mcjun coding sequence is fused in phase to the N-terminal S. cerevisiae GALbd domain.
The S. cerevisiae GALbd is composed of the first 147 codons from GAL4, containing the nuclear localization signal.
The GALbd-mcjun fusion protein can be used as a bait protein in two-hybrid screening.
The nucleotide sequence of the mcjun cDNA corresponds with the Genbank accession number NNM_010591.1.
EMBL Accession number: NM_010591.1, view at GenBank
Latest sequence update: 21/10/2005
Sequence detail:
Nucleotide sequence of the GALbd-mcjun fusion gene:

                                                  ------------------ GALbd ----------
                                                  2
5' ... ATATTCACTG.ATG.GAC.TCC.CAG.CAG.CCA.GAT.CTG.AAG.CTA.CTG.TCT.TCT ... CAG.TTG.ACT
                  Met Asp Ser Gln Gln Pro Asp Leu Lys Leu Leu Ser Ser     Gln Leu Thr
                  ***                   BglII                               HindII


      ------>                                             ------------------ mcjun --
          147                                             1
      GTA.TCG.CCG.GAA.TTC.CCG.GGT.GTC.GAC.CAG.GAC.CGT.TCT.ATG.ACT.GCA.AAG.ATG ... TTG
      Val Ser Pro Glu Phe Pro Gly Val Asp Gln Asp Arg Ser Met Thr Ala Lys Met     Leu
                  EcoRI AvaI      HindII    AvaII         ***
                        SmaI      SalI

      -------------->
              334
      CAA.ACG.TTT.TGA.GAACAGACTG ... 3'
      Gln Thr Phe +++


***: start codon.
+++: termination codon.
Punctuation indicates reading frame.
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AvaI/XmnI, BamHI, BglI/SalI, BglII/PvuII, BssHII/EcoRI, KpnI/NcoI and NruI/ScaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr W. Vanden Berghe(1) and Prof. Dr G. Haegeman(1). It was constructed by S. Plaisance(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pAB-jun (LMBP 5040) is available at BCCM/GeneCorner. This plasmid was deposited by Dr W. Vanden Berghe and Prof. Dr G. Haegeman.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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