Last data update: 24 January 2024 16:39 CET
Plasmid name: pAB-jun (LMBP 5040)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5040.gb
(View with Genome Compiler) p5040.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) Mouse c-jun oncogene cDNA (mcjun) |
| Promoter: | Rous sarcoma virus long terminal repeat (RSV-LTR) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pABgal |
| Further information: | The plasmid was constructed by inserting the mouse c-jun oncogene (mcjun) cDNA into the PvuII opened pAB-gal vector. As a result, the mcjun coding sequence is fused in phase to the N-terminal S. cerevisiae GALbd domain. The S. cerevisiae GALbd is composed of the first 147 codons from GAL4, containing the nuclear localization signal. The GALbd-mcjun fusion protein can be used as a bait protein in two-hybrid screening. The nucleotide sequence of the mcjun cDNA corresponds with the Genbank accession number NNM_010591.1. |
| EMBL Accession number: | NM_010591.1, view at GenBank |
| Latest sequence update: | 21/10/2005 |
| Sequence detail: | Nucleotide sequence of the GALbd-mcjun fusion gene:
------------------ GALbd ----------
2
5' ... ATATTCACTG.ATG.GAC.TCC.CAG.CAG.CCA.GAT.CTG.AAG.CTA.CTG.TCT.TCT ... CAG.TTG.ACT
Met Asp Ser Gln Gln Pro Asp Leu Lys Leu Leu Ser Ser Gln Leu Thr
*** BglII HindII
------> ------------------ mcjun --
147 1
GTA.TCG.CCG.GAA.TTC.CCG.GGT.GTC.GAC.CAG.GAC.CGT.TCT.ATG.ACT.GCA.AAG.ATG ... TTG
Val Ser Pro Glu Phe Pro Gly Val Asp Gln Asp Arg Ser Met Thr Ala Lys Met Leu
EcoRI AvaI HindII AvaII ***
SmaI SalI
-------------->
334
CAA.ACG.TTT.TGA.GAACAGACTG ... 3'
Gln Thr Phe +++
***: start codon.
+++: termination codon.
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AvaI/XmnI, BamHI, BglI/SalI, BglII/PvuII, BssHII/EcoRI, KpnI/NcoI and NruI/ScaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr W. Vanden Berghe(1) and Prof. Dr G. Haegeman(1). It was constructed by S. Plaisance(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pAB-jun (LMBP 5040) is available at BCCM/GeneCorner. This plasmid was deposited by Dr W. Vanden Berghe and Prof. Dr G. Haegeman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.