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GeneCorner plasmid details

Last data update: 23 October 2020 04:27 CEST

Plasmid name: pAB130 (LMBP 3112)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse E-cadherin cDNA (E-cad, CDH1); fragment
Promoter: Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pMECAD; pBluescript
Further information: This plasmid was constructed by inserting the SacI-XbaI fragment of pMECAD, containing a 616 bp fragment of the coding sequence of mouse E-cadherin, between the SacI and XbaI sites of a pBluescript vector.
The plasmid can be used to produce RNA probes.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
EMBL Accession number: -
Latest sequence update: 27/01/1997
Authenticity test: By lack of sequence data, it was not possible to perform the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M.J. Bussemakers(1).
(1) Urological Research Laboratory, Radboud University Hospital, Nijmegen, The Netherlands
Plasmid reference: Bussemakers et al., Cancer Res. 52 (1992), 2916-2922 [PMID: 1581909]
Related plasmid reference: Nagafuchi et al., Nature 329 (1987), 341-343 [PMID: 3498123]
Restricted distribution: - BCCM MTA
- A brief description of the enduser's research is asked by the depositor.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pAB130 (LMBP 3112) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M.J. Bussemakers and was published in Bussemakers et al., 1992.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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