Last data update: 24 January 2024 16:39 CET
Plasmid name: pAB130 (LMBP 3112)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Mouse cadherin 1 cDNA (Cdh1, Ecad, E-cadherin, L-CAM, UM, uvomorulin, GeneID 12550); fragment |
Promoter: | Phage T7 gene 10 promoter (T7g10) Phage T3 promoter Escherichia coli lac operon promoter |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
Host range: | Escherichia coli |
Parental clone: | pMECAD; pBluescript |
Further information: | This plasmid was constructed by inserting the SacI-XbaI fragment of pMECAD, containing a 616 bp fragment of the coding sequence of mouse E-cadherin, between the SacI and XbaI sites of a pBluescript vector. The plasmid can be used to produce RNA probes. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. |
EMBL Accession number: | - |
Latest sequence update: | 27/01/1997 |
Authenticity test: | By lack of sequence data, it was not possible to perform the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr M.J. Bussemakers(1). (1) Urological Research Laboratory, Radboud University Hospital, Nijmegen, The Netherlands |
Plasmid reference: | Bussemakers et al., Cancer Res. 52 (1992), 2916-2922 [PMID: 1581909] |
Related plasmid reference: | Nagafuchi et al., Nature 329 (1987), 341-343 [PMID: 3498123] |
Restricted distribution: | - BCCM MTA - A brief description of the enduser's research is asked by the depositor. |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pAB130 (LMBP 3112) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M.J. Bussemakers and was published in Bussemakers et al., 1992. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.