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GeneCorner plasmid details

Last data update: 27 October 2020 04:27 CET

Plasmid name: pAB29 (LMBP 9278)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Yersinia enterocolitica anti-host protein T gene (yopT)
Bordatella pertussis calmodulin-dependent adenylate cyclase cDNA (cyaA); fragment (cyaA')
Promoter: Yersinia enterocolitica anti-host protein E promoter (yopE)
Ribosome
binding site:
-
Terminator: -
Selection marker: Chloramphenicol (cam)
Replicon: Broad-host-range plasmid RK2 origin of transfer (oriT)
Escherichia coli plasmid p15A origin
Host range: Escherichia coli
Gram-negative bacterial strains
Parental clone: pAB28; pMSL56
Further information: The plasmid was constructed by PCR amplifying the first 124 codons of Y. enterocolitica yopT from pAB28, and fusing it to B. pertussis cyaA'. This hybrid gene was cloned downstream from the yopE promoter in the pACYC184-derived pMSL56 vector.
This is a reporter plasmid for YopT delivery inside macrophages via the adenylate cyclase reporter strategy.
B. pertussis cyaA' is a fragment of the B. pertussis cyaA gene, containing nucleotides 4 to 1197.
Other name of the plasmid is pMSL56 PyopE yopT124-cyaA'+.
EMBL Accession number: -
Latest sequence update: 03/07/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr A. Boland(2).
(1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium
(2) Microbial Pathogenesis Unit, Institute of Cellular and Molecular Pathology, Catholic University of Louvain, Brussels, Belgium
Plasmid reference: Iriarte et al., Mol. Microbiol. 29 (1998), 915-929 [PMID: 9723929]
Related plasmid reference: Sory et al., Mol. Microbiol. 14 (1994), 583-594 [PMID: 7885236]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 S17-1
Host reference: -
Related host reference: Simon et al., Biotechnology 1 (1983), 784-791
Cultivation medium: LB-Lennox + chloramphenicol (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pAB29 (LMBP 9278) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Cornelis and was published in Iriarte et al., 1998.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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