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GeneCorner plasmid details

Last data update: 27 October 2020 04:27 CET

Plasmid name: pAB4052 (LMBP 9292)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Yersinia enterocolitica arsenite pump transmembrane protein gene (arsB)
Yersinia enterocolitica arsenate reductase gene (arsC)
Yersinia enterocolitica arsenic resistance protein gene (arsH)
Yersinia enterocolitica arsenite inducible repressor gene (arsR)
Yersinia enterocolitica low calcium response protein genes (lcrD, lcrG, lcrR and lcrV)
Yersinia enterocolitica non-specific endonuclease gene (nuc)
Yersinia enterocolitica positive transcriptional regulator gene (pprA)
Yersinia enterocolitica plasmid pYV replication protein genes (repA and repB)
Yersinia enterocolitica plasmid pYV replication protein gene (repB)
Yersinia enterocolitica transcriptional regulator gene (spyA)
Yersinia enterocolitica DNA binding protein gene (spyB)
Yersinia enterocolitica specific yop chaperone gene (sycD, sycE, sycH, sycN and sycT)
Yersinia enterocolitica transposase gene (tnpA)
Yersinia enterocolitica resolvase gene (tnpR)
Yersinia enterocolitica DNA helicase gene (traI)
Yersinia enterocolitica IncF plasmid conjugative transfer pilin acetylase gene (traX)
Yersinia enterocolitica yop release control protein gene (tyeA)
Yersinia enterocolitica transcriptional activator gene (virF)
Yersinia enterocolitica lipoprotein gene (virG)
Yersinia enterocolitica outer membrane adhesin protein gene (yadA)
Yersinia enterocolitica surface exclusion protein gene (ylpA)
Yersinia enterocolitica integral membrane protein gene (yomA)
Yersinia enterocolitica anti-host protein genes (yopB, yopD, yopE, yopH, yopM, yopN, yopP, yopQ and yopT)
Yersinia enterocolitica anti-host protein E gene (yopE); inactive mutant (yopE21)
Yersinia enterocolitica secretion protein genes (yscA, yscB, yscC, yscD, yscE, yscF, yscG, yscH, yscI, yscJ, yscK, yscL, yscM1, yscM2, yscN, yscO, yscP, yscQ, yscR, yscS, yscT, yscU, yscV, yscW, yscX and yscY)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Arsenic (ars)
Replicon: Yersinia enterocolitica plasmid pYV40 ori
Host range: Yersinia enterocolitica
Parental clone: pYV40; pPW52
Further information: The plasmid was constructed via allelic exchange between pYV40 and pPW52, containing a mutant version of Y. enterocolitica yopE, designated yopE21.
Y. enterocolitica yopE21 has a translational frame-shift following a 4 bp insert between codons 3 - 4. An additional BglII site is present at this place.
This plasmid was the first step towards the ΔHOPEMT multimutant strain (pYV40 yopE21 yopHΔ1-352 yopOΔ65-558 yopP23 yopM23 yopT135).
Other names of the plasmid are pYV40 yopE and pYV40 yopE21.
EMBL Accession number: -
Latest sequence update: 03/07/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr A. Boland(2).
(1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium
(2) Microbial Pathogenesis Unit, Institute of Cellular and Molecular Pathology, Catholic University of Louvain, Brussels, Belgium
Plasmid reference: Mills et al., Proc. Natl. Acad. Sci. U.S.A. 94 (1997), 12638-12643 [PMID: 9356502]
Related plasmid reference: Sory et al., Proc. Natl. Acad. Sci. U.S.A. 92 (1995), 11998-12002 [PMID: 8618831]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Yersinia enterocolitica E40
Host reference: -
Cultivation medium: BHI + arsenic (400 μM) + nalidixic acid (35 μg/ml)
Cultivation temperature: 20°C
Biosafety level: L2
Cultivation remark: Cultivate on LB agar plates, or in liquid BHI.
Other culture collection numbers: -

Refer in your Materials and Methods:

pAB4052 (LMBP 9292) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Cornelis and was published in Mills et al., 1997.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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