Last data update: 24 January 2024 16:39 CET
Plasmid name: pABgal (LMBP 3660)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3660.gb
(View with Genome Compiler) p3660.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) |
| Promoter: | Rous sarcoma virus long terminal repeat (RSV-LTR) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pRSVCAT; pSV2CAT |
| Further information: | This plasmid can be used for fusion of a cDNA of interest to the GAL4 DNA-binding domain (GALbd) to study, in mammalian cells, the effect of the fusion protein on a reporter gene with a GAL4 binding site as promoter (e.g. pUAStkCAT). The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727369.1. The nucleotide sequence of pABgal has been compiled in accordance with the description in Baniahmad et al. (1992) and in Giguère et al. (1986). The 3' region of GALbd and the multicloning site have been sequenced at the Department of Biomedical Molecular Biology (Ghent University, Belgium) with the use of oligo 175. The nucleotide sequence upstream of GALbd (from nucleotide position 1381 up to and including 1950) has been analysed by BCCM/GeneCorner on collection number LMBP 3660. Other names of the plasmid are Gal4/3 and pGalAB. |
| EMBL Accession number: | LT727369.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 22/09/2003 |
| Sequence detail: | Nucleotide sequence at the start of the Saccharomyces cerevisiae GAL4 DNA-binding domain:
-------------- GALbd -------------->
2 5 10
5' ... TCACTG.ATG.GAC.TCC.CAG.CAG.CCA.GAT.CTG.AAG.CTA.CTG.TCT.TCT.ATC.GAA.CAA.GCA ... 3'
Met Asp Ser Gln Gln Pro Asp Leu Lys Leu Leu Ser Ser Ile Glu Gln Ala
*** BglII
***: start codon.
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI and HindIII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr A. Baniahmad(1). (1) Genetisches Institut, Giessen, Germany |
| Plasmid reference: | Baniahmad et al., EMBO J. 11 (1992), 1015-1023 [PMID: 1347744] |
| Related plasmid reference: | Giguère et al., Cell 46 (1986), 645-652 [PMID: 3742595] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pABgal (LMBP 3660) is available at BCCM/GeneCorner. This plasmid was deposited by Dr A. Baniahmad and was published in Baniahmad et al., 1992. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.