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GeneCorner plasmid details

Last data update: 29 October 2020 04:15 CET

Plasmid name: pABgal (LMBP 3660)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p3660.gb (View with Genome Compiler)
p3660.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd)
Promoter: Rous sarcoma virus long terminal repeat (RSV-LTR)
Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pRSVCAT; pSV2CAT
Further information: This plasmid can be used for fusion of a cDNA of interest to the GAL4 DNA-binding domain (GALbd) to study, in mammalian cells, the effect of the fusion protein on a reporter gene with a GAL4 binding site as promoter (e.g. pUAStkCAT).
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727369.1.
The nucleotide sequence of pABgal has been compiled in accordance with the description in Baniahmad et al. (1992) and in Giguère et al. (1986). The 3' region of GALbd and the multicloning site have been sequenced at the Department of Biomedical Molecular Biology (Ghent University, Belgium) with the use of oligo 175. The nucleotide sequence upstream of GALbd (from nucleotide position 1381 up to and including 1950) has been analysed by BCCM/GeneCorner on collection number LMBP 3660.
Other names of the plasmid are Gal4/3 and pGalAB.
EMBL Accession number: LT727369.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 22/09/2003
Sequence detail:
Nucleotide sequence at the start of the Saccharomyces cerevisiae GAL4 DNA-binding domain:

                                              -------------- GALbd -------------->
                                              2           5                   10 
5' ... TCACTG.ATG.GAC.TCC.CAG.CAG.CCA.GAT.CTG.AAG.CTA.CTG.TCT.TCT.ATC.GAA.CAA.GCA ... 3'
              Met Asp Ser Gln Gln Pro Asp Leu Lys Leu Leu Ser Ser Ile Glu Gln Ala
              ***                   BglII


***: start codon.
Punctuation indicates reading frame.
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI and HindIII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr A. Baniahmad(1).
(1) Genetisches Institut, Giessen, Germany
Plasmid reference: Baniahmad et al., EMBO J. 11 (1992), 1015-1023 [PMID: 1347744]
Related plasmid reference: Giguère et al., Cell 46 (1986), 645-652 [PMID: 3742595]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pABgal (LMBP 3660) is available at BCCM/GeneCorner. This plasmid was deposited by Dr A. Baniahmad and was published in Baniahmad et al., 1992.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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