Last data update: 24 January 2024 16:39 CET
Plasmid name: pABgal94-hCNOT2 (LMBP 9789)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human CCR4-NOT transcription complex subunit 2 cDNA (CNOT2, NOT2H, GeneID 4848) Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) |
| Promoter: | Rous sarcoma virus long terminal repeat (RSV-LTR) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Simian virus 40 bidirectional origin (SV40) Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pABgal94 |
| Further information: | The plasmid was constructed by cloning the human CNOT2 coding sequence into the XmaI/BamHI opened pABgal94 vector. Other name of the plasmid is Gal4-CNOT2. |
| EMBL Accession number: | - |
| Latest sequence update: | 22/01/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr L. Schmitz(1). (1) Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-University, Giessen, Germany |
| Plasmid reference: | - |
| Related plasmid reference: | Baniahmad et al., Mol. Cell. Biol. 15 (1995), 76-86 [PMID: 7799971] [DOI: 10.1128/MCB.15.1.76] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pABgal94-hCNOT2 (LMBP 9789) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr L. Schmitz. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.