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GeneCorner plasmid details

Last data update: 22 October 2020 04:25 CEST

Plasmid name: pACCMVpLpA-E-hRac1-CA (LMBP 4885)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4885.gb (View with Genome Compiler)
p4885.txt
p4885.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human adenovirus 5 genome (Ad5); fragment
Human Rac family small GTPase 1 cDNA (RAC1, Rac-1, TC-25, GeneID 5879); constitutive active G12V mutant
E-tag; N-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Human adenovirus 5 origin (Ad5)
Host range: Escherichia coli
Mammalian cells
Parental clone: pCAGGS-EhRAC1-DP; pACCMVpLpA
Further information: The plasmid was constructed by inserting the E-tagged, constitutive active or dominant positive mutant of the human RAC1 cDNA as an EcoRI-KpnI fragment from pCAGGS-EhRAC1-DP into the EcoRI-KpnI opened pACCMVpLpA vector.
This adenoviral shuttle plasmid is used in combination with e.g. pJM17 to generate recombinant Ad5 viruses.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727055.
The nucleotide sequence of the Ad5 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X02996.1.
The nucleotide sequence of the wt RAC1 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF498964.1.
Other name of the plasmid is pACCMVpLpA-Rac1CA.
EMBL Accession number: X02996.1, view at EMBL, GenBank, DDBJ
AF498964.1, view at EMBL, GenBank, DDBJ
LT727055, view at EMBL, GenBank, DDBJ
Latest sequence update: 23/03/2009
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglII, BstZI/SpeI, HincII, NcoI and StuI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed by Dr P. Schotte(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Becker et al., Methods Cell Biol. 43 (1994), 161-189 [PMID: 7823861]
Gomez-Foix et al., J. Biol. Chem. 267 (1992), 25129-25134 [PMID: 1334082]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pACCMVpLpA-E-hRac1-CA (LMBP 4885) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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