Last data update: 24 January 2024 16:39 CET
Plasmid name: pACM19 (LMBP 9065)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9065.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Klebsiella oxytoca class I integrase gene (intl1); fragment Klebsiella oxytoca quaternary ammonium compound resistance protein gene (qacEdelta1); fragment Open reading frame (ORF); function unknown |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Kanamycin (kan; amikacin; tobramycin) Streptomycin (Sm; spectinomycin (Sp)) Gentamicin (Gm; tobramycin (tob)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUC19; pACM1 |
| Further information: | The plasmid was constructed by inserting the HindIII/EcoRI fragment of Klebsiella oxytoca plasmid pACM1 into pUC19, and deleting the SmaI fragments and NruI fragments. The NruI site was lost in religation. The plasmid contains the complete integron cassettes aacA1, conferring resistance to antibiotics containing the purpurosamine ring, including amikacin, kanamycin and tobramycin, aacA4, conferring resistance to gentamicin and tobramycin and aadA1, conferring resistance to streptomycin and spectinomycin. |
| EMBL Accession number: | - |
| Latest sequence update: | 31/03/2014 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr K. Preston(1). (1) Center for Functional Genomics, University at Albany, Rensselaer, NY, USA |
| Plasmid reference: | Preston et al., Plasmid 37 (1997), 105-118 [PMID: 9169202] [DOI: 10.1006/plas.1997.1284] |
| Related plasmid reference: | Preston et al., Plasmid 76 (2014), 54-65 [PMID: 25291385] [DOI: 10.1016/j.plasmid.2014.08.005] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pACM19 (LMBP 9065) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. Preston and was published in Preston et al., 1997. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.