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GeneCorner plasmid details

Last data update: 26 October 2020 04:25 CET

Plasmid name: pACM19 (LMBP 9065)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9065.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Klebsiella oxytoca class I integrase gene (intl1); fragment
Klebsiella oxytoca quaternary ammonium compound resistance protein gene (qacEdelta1); fragment
Open reading frame (ORF); function unknown
Promoter: Escherichia coli lac operon promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: -
Selection marker: Ampicillin (amp)
Kanamycin (kan; amikacin; tobramycin)
Streptomycin (Sm; spectinomycin (Sp))
Gentamicin (Gm; tobramycin (tob))
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pUC19; pACM1
Further information: The plasmid was constructed by inserting the HindIII/EcoRI fragment of Klebsiella oxytoca plasmid pACM1 into pUC19, and deleting the SmaI fragments and NruI fragments. The NruI site was lost in religation.
The plasmid contains the complete integron cassettes aacA1, conferring resistance to antibiotics containing the purpurosamine ring, including amikacin, kanamycin and tobramycin, aacA4, conferring resistance to gentamicin and tobramycin and aadA1, conferring resistance to streptomycin and spectinomycin.
EMBL Accession number: -
Latest sequence update: 31/03/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. Preston(1).
(1) Center for Functional Genomics, University at Albany, Rensselaer, NY, USA
Plasmid reference: Preston et al., Plasmid 37 (1997), 105-118 [PMID: 9169202]
Related plasmid reference: Preston et al., Plasmid 76 (2014), 54-65 [PMID: 25291385] [DOI: 10.1016/j.plasmid.2014.08.005]
Restricted distribution: - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
- BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pACM19 (LMBP 9065) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. Preston and was published in Preston et al., 1997.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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