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GeneCorner plasmid details

Last data update: 29 November 2020 04:28 CET

Plasmid name: pACM44 (LMBP 9067)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9067.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Klebsiella oxytoca class I integrase gene (intl1); fragment
Promoter: Escherichia coli lac operon promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: -
Selection marker: Ampicillin (amp)
Streptomycin (Sm; spectinomycin (Sp))
Trimethoprim (tmp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pUC19; pACM1
Further information: The plasmid was constructed via several subcloning steps, resulting in an AspI (blunt)/NruI fragment of Klebsiella oxytoca plasmid pACM1 in the EcoRI (blunt)/SmaI opened pUC19 vector.
The plasmid contains the complete integron cassettes aadA1, conferring resistance to streptomycin and spectinomycin and dfrA1, conferring resistance to trimethoprim.
EMBL Accession number: -
Latest sequence update: 31/03/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. Preston(1).
(1) Center for Functional Genomics, University at Albany, Rensselaer, NY, USA
Plasmid reference: Preston et al., Plasmid 37 (1997), 105-118 [PMID: 9169202]
Related plasmid reference: Preston et al., Plasmid 76 (2014), 54-65 [PMID: 25291385] [DOI: 10.1016/j.plasmid.2014.08.005]
Restricted distribution: - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
- BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pACM44 (LMBP 9067) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. Preston and was published in Preston et al., 1997.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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