Last data update: 25 October 2020 04:11 CET
Plasmid name: pACT-Tax1BP1 (LMBP 6917)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human T-cell leukemia virus type I (HTLV-1) Tax 1 binding protein 1 cDNA (TXBP15-1; TXBP151; TAX1BP1)
|Promoter:||Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Simian virus 40 early promoter (SV40 early)
Phage T3 promoter
|Selection marker:||Neomycin (neo; G418; kanamycin (kan))
|Replicon:||Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Escherichia coli plasmid pMB1 origin
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Parental clone:||pACT; pCAGGS-FLAG-Tax1BP1|
|Further information:||The plasmid was constructed via PCR amplification of TAX1BP1 with pCAGGS-FLAG-Tax1BP1 as a template. The TAX1BP1 cds was ligated as an NcoI/EcoRI fragment into the NcoI/EcoRI opened pACT vector.
The nucleotide sequence of the pACT vector corresponds with the EMBL Nucleotide Sequence Database accession number AF264723.1.
The nucleotide sequence of the TAX1BP1 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number U33821.2.
|EMBL Accession number:||AF264723.1, view at EMBL, GenBank, DDBJ
U33821.2, view at EMBL, GenBank, DDBJ
|Latest sequence update:||23/02/2011|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by K. Verhelst(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pACT-Tax1BP1 (LMBP 6917) is available at BCCM/GeneCorner. This plasmid was deposited by K. Verhelst and Prof. Dr R. Beyaert .|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.