Last data update: 24 January 2024 16:39 CET
Plasmid name: pACT-Tax1BP1 (LMBP 6917)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p6917.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human Tax1 binding protein 1 cDNA (TAX1BP1, T6BP, TXBP151, GeneID 8887) |
Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Simian virus 40 early promoter (SV40 early) Phage T3 promoter |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Neomycin (neo; G418; kanamycin (kan)) Ampicillin (amp) |
Replicon: | Phage f1 origin Simian virus 40 bidirectional origin (SV40) Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells Yeast |
Parental clone: | pACT; pCAGGS-FLAG-Tax1BP1 |
Further information: | The plasmid was constructed via PCR amplification of TAX1BP1 with pCAGGS-FLAG-Tax1BP1 as a template. The TAX1BP1 cds was ligated as an NcoI/EcoRI fragment into the NcoI/EcoRI opened pACT vector. The nucleotide sequence of the pACT vector corresponds with the EMBL Nucleotide Sequence Database accession number AF264723.1. The nucleotide sequence of the TAX1BP1 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number U33821.2. |
EMBL Accession number: | AF264723.1, view at EMBL, GenBank, DDBJ U33821.2, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 23/02/2011 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by K. Verhelst(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pACT-Tax1BP1 (LMBP 6917) is available at BCCM/GeneCorner. This plasmid was deposited by K. Verhelst and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.