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GeneCorner plasmid details

Last data update: 25 October 2020 04:11 CET

Plasmid name: pACT-Tax1BP1 (LMBP 6917)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p6917.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human T-cell leukemia virus type I (HTLV-1) Tax 1 binding protein 1 cDNA (TXBP15-1; TXBP151; TAX1BP1)
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Simian virus 40 early promoter (SV40 early)
Phage T3 promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Neomycin (neo; G418; kanamycin (kan))
Ampicillin (amp)
Replicon: Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Yeast
Parental clone: pACT; pCAGGS-FLAG-Tax1BP1
Further information: The plasmid was constructed via PCR amplification of TAX1BP1 with pCAGGS-FLAG-Tax1BP1 as a template. The TAX1BP1 cds was ligated as an NcoI/EcoRI fragment into the NcoI/EcoRI opened pACT vector.
The nucleotide sequence of the pACT vector corresponds with the EMBL Nucleotide Sequence Database accession number AF264723.1.
The nucleotide sequence of the TAX1BP1 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number U33821.2.
EMBL Accession number: AF264723.1, view at EMBL, GenBank, DDBJ
U33821.2, view at EMBL, GenBank, DDBJ
Latest sequence update: 23/02/2011
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by K. Verhelst(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pACT-Tax1BP1 (LMBP 6917) is available at BCCM/GeneCorner. This plasmid was deposited by K. Verhelst and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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