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GeneCorner plasmid details

Last data update: 30 October 2020 04:11 CET

Plasmid name: pACT2-D4.4 (LMBP 11148)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human dopamine receptor D4 cDNA (DRD4, GeneID 1815); variant DRD4.4
Saccharomyces cerevisiae GAL4 activation domain (GALad); N-terminal
Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal
Promoter: Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1)
Ribosome
binding site:
-
Terminator: Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1)
Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator
Selection marker: Ampicillin (amp)
Saccharomyces cerevisiae LEU2; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Saccharomyces cerevisiae 2 micron plasmid origin (2μ)
Host range: Escherichia coli
Saccharomyces cerevisiae; leu2(-)
Parental clone: pACT2
Further information: The plasmid was constructed by cloning the DRD4 variant into the pACT2 vector.
The nucleotide sequence of the pACT2 vector corresponds with the EMBL Nucleotide Sequence Database accession number U29899.1.
EMBL Accession number: U29899.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 09/08/2019
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr K. Van Craenenbroeck(1).
(1) Laboratory for GPCR Expression and Signal Transduction, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pACT2-D4.4 (LMBP 11148) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. Van Craenenbroeck.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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