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GeneCorner plasmid details

Last data update: 25 October 2020 04:11 CET

Plasmid name: pACT2-IKKbeta (LMBP 5494)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human NF-κB inhibitor kinase β cDNA (IKKβ, IKBKB)
Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal
Saccharomyces cerevisiae GAL4 activation domain (GALad); N-terminal
Promoter: Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1)
Ribosome
binding site:
-
Terminator: Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1)
Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator
Selection marker: Ampicillin (amp)
LEU2; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Saccharomyces cerevisiae 2 micron plasmid origin (2μ)
Host range: Escherichia coli
Saccharomyces cerevisiae; leu2(-)
Parental clone: pACT2-IKKβΔ; pCDA-IKKβ
Further information: The nucleotide sequence of the pACT2 vector corresponds with the EMBL Nucleotide Sequence Database accession number U29899.1.
EMBL Accession number: U29899.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 30/08/2007
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. Heyninck(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pACT2-IKKbeta (LMBP 5494) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. Heyninck and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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