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GeneCorner plasmid details

Last data update: 30 October 2020 04:11 CET

Plasmid name: pACTyEGFPTU (LMBP 4101)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p4101.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Aequorea victoria green fluorescent protein DNA (GFP); yeast enhanced sequence (yEGFP)
Promoter: Candida albicans actin promoter (ACT1)
Ribosome
binding site:
-
Terminator: Candida albicans alpha-glucosidase terminator (MAL2)
Selection marker: Ampicillin (amp)
Candida albicans URA3; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Candida albicans; ura3(-), integrative
Parental clone: pACT1PyEGFP3T-1; pGAL1PNiST-1
Further information: This plasmid was constructed by inserting a 1424 bp ScaI/FspI fragment from pGAL1PNiST-1, containing the Candida albicans URA3 coding sequence, in the SmaI site of pACT1PyEGFP3T-1. The orientation of the inserted fragment is antisense relative to the ampicillin resistance gene.
pACTyEGFPTU is a stable integrative vector for C. albicans and shows constitutive expression of yeast enhanced green fluorescent protein (yEGFP3).
pACTyEGFPTU can be used as a positive control in fluorescence-activated cell sorting (FACS) of C. albicans GFP expression strains.
The origin of the sequence between the MAL2 terminator and the yEGFP3 sequence is unknown.
The nucleotide sequence of the CaURA3 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X14198.1. HOWEVER, THERE MIGHT BE CORRESPONDENCE WITH AF109400.1: see LMBP 4100.
Other name of the plasmid is pACT1PyEGFP3TU-1.
EMBL Accession number: X14198.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/07/2000
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr N. Callewaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Cormack et al., Microbiology 143 (1997), 303-311 [PMID: 9043107]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pACTyEGFPTU (LMBP 4101) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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