Last data update: 24 January 2024 16:39 CET
Plasmid name: pACTyEGFPTU (LMBP 4101)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p4101.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Aequorea victoria green fluorescent protein DNA (GFP); yeast enhanced sequence (yEGFP) |
| Promoter: | Candida albicans actin promoter (ACT1) |
| Ribosome binding site: |
- |
| Terminator: | Candida albicans alpha-glucosidase terminator (MAL2) |
| Selection marker: | Ampicillin (amp) Candida albicans URA3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Candida albicans; ura3(-), integrative |
| Parental clone: | pACT1PyEGFP3T-1; pGAL1PNiST-1 |
| Further information: | This plasmid was constructed by inserting a 1424 bp ScaI/FspI fragment from pGAL1PNiST-1, containing the Candida albicans URA3 coding sequence, in the SmaI site of pACT1PyEGFP3T-1. The orientation of the inserted fragment is antisense relative to the ampicillin resistance gene. pACTyEGFPTU is a stable integrative vector for C. albicans and shows constitutive expression of yeast enhanced green fluorescent protein (yEGFP3). pACTyEGFPTU can be used as a positive control in fluorescence-activated cell sorting (FACS) of C. albicans GFP expression strains. The origin of the sequence between the MAL2 terminator and the yEGFP3 sequence is unknown. The nucleotide sequence of the CaURA3 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X14198.1. HOWEVER, THERE MIGHT BE CORRESPONDENCE WITH AF109400.1: see LMBP 4100. Other name of the plasmid is pACT1PyEGFP3TU-1. |
| EMBL Accession number: | X14198.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 03/07/2000 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Cormack et al., Microbiology 143 (1997), 303-311 [PMID: 9043107] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pACTyEGFPTU (LMBP 4101) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.