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GeneCorner plasmid details

Last data update: 22 October 2020 04:25 CEST

Plasmid name: pACTyEGFPTUHD1 (LMBP 4178)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p4178.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Aequorea victoria green fluorescent protein DNA (GFP); yeast enhanced sequence (yEGFP)
Candida albicans histidine 1 selectable marker gene (HIS1); fragment
Promoter: Candida albicans actin promoter (ACT1)
Ribosome
binding site:
-
Terminator: Candida albicans alpha-glucosidase terminator (MAL2)
Selection marker: Ampicillin (amp)
Candida albicans URA3; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Candida albicans; ura3(-), integrative
Parental clone: pACTyEGFPTU; pUCCaHIS1-1
Further information: This plasmid was constructed by inserting a 781 bp PstI (blunted with S1 nuclease) - EcoRI (filled in with T4 DNA polymerase) fragment of pUCCaHIS1-1, containing part of the Candida albicans histidine 1 selectable marker gene, in the PvuII site of pACTyEGFPTU. The orientation of the inserted fragment is antisense relative to the ampicillin resistance gene.
pACTyEGFPTUHD1 is a stable integrative vector and allows integration of the linearized vector into the Candida albicans genome via homologous recombination at the HIS1 locus.
The origin of the sequence between the MAL2 terminator and the yEGFP3 sequence is unknown.
The nucleotide sequence of the CaURA3 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X14198.1. HOWEVER, THERE MIGHT BE CORRESPONDENCE WITH AF109400.1: see LMBP 4100.
Other name of the plasmid is pACT1PyEGFP3TUHD-1.
EMBL Accession number: X14198.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 29/08/2000
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr N. Callewaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pACTyEGFPTUHD1 (LMBP 4178) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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