Last data update: 24 January 2024 16:39 CET
Plasmid name: pACTyEGFPTUHD2 (LMBP 4179)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p4179.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Aequorea victoria green fluorescent protein DNA (GFP); yeast enhanced sequence (yEGFP) Candida albicans histidine 1 selectable marker gene (HIS1); fragment |
Promoter: | Candida albicans actin promoter (ACT1) |
Ribosome binding site: |
- |
Terminator: | Candida albicans alpha-glucosidase terminator (MAL2) |
Selection marker: | Ampicillin (amp) Candida albicans URA3; auxotrophic |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli Candida albicans; ura3(-), integrative |
Parental clone: | pACTyEGFPTU; pUCCaHIS1-1 |
Further information: | This plasmid was constructed by inserting a 781 bp PstI (blunted with S1 nuclease) - EcoRI (filled in with T4 DNA polymerase) fragment of pUCCaHIS1-1, containing part of the Candida albicans histidine 1 selectable marker gene, in the PvuII site of pACTyEGFPTU. The orientation of the inserted fragment is sense relative to the ampicillin resistance gene. pACTyEGFPTUHD2 is a stable integrative vector and allows integration of the linearized vector into the Candida albicans genome via homologous recombination at the HIS1 locus. The origin of the sequence between the MAL2 terminator and the yEGFP3 sequence is unknown. The nucleotide sequence of the CaURA3 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X14198.1. HOWEVER, THERE MIGHT BE CORRESPONDENCE WITH AF109400.1: see LMBP 4100. Other name of the plasmid is pACT1PyEGFP3TUHD-2. |
EMBL Accession number: | X14198.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 29/08/2000 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pACTyEGFPTUHD2 (LMBP 4179) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.