GeneCorner plasmid details

Last data update: 16 June 2021 13:31 CEST

Plasmid name: pACTyEGFPTUMD1 (LMBP 4180)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence:
analysis results


Cloned DNA: Aequorea victoria green fluorescent protein DNA (GFP); yeast enhanced sequence (yEGFP)
Candida albicans bifunctional cysteine synthase/O-acetylhomoserine aminocarboxypropyltransferase genomic DNA (MET15, GeneID 3645031); fragment
Promoter: Candida albicans actin promoter (ACT1)
binding site:
Terminator: Candida albicans alpha-glucosidase terminator (MAL2)
Candida albicans O-acetylhomoserine O-acetylserine sulfhydrylase terminator (CaMET15)
Selection marker: Ampicillin (amp)
Candida albicans URA3; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Candida albicans; ura3(-), integrative
Parental clone: pACTyEGFPTU; pCaMET15
Further information: This plasmid was constructed as follows: pCaMET15 was cleaved with BanII (blunted with T4 DNA polymerase)/AatII and the resulting 4044 bp fragment (containing part of the Candida albicans O-acetylhomoserine O-acetylserine sulfhydrylase (CaMET15) genomic DNA, the origin of replication and the ampicillin resistance gene) was ligated into the XmnI/AatII opened pACTyEGFPTU vector.
The orientation of the MET15 coding sequence is sense relative to the URA3 gene.
pACT1EGFPTUMD1 is a stable integrative vector and allows integration of the linearized vector into the Candida albicans genome via homologous recombination at the MET15 locus.
The origin of the sequence between the MAL2 terminator and the yEGFP3 sequence is unknown.
The nucleotide sequence of the CaMET15 genomic DNA corresponds with the EMBL Nucleotide Sequence Database accession number AF188275.1.
The nucleotide sequence of the CaURA3 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X14198.1. HOWEVER, THERE MIGHT BE CORRESPONDENCE WITH AF109400.1: see LMBP 4100.
Other name of the plasmid is pACT1PyEGFP3TUMD-1.
EMBL Accession number: X14198.1, view at EMBL, GenBank, DDBJ
AF188275.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 29/08/2000
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr N. Callewaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pACTyEGFPTUMD1 (LMBP 4180) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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