Last data update: 24 January 2024 16:39 CET
Plasmid name: pACYC177 (LMBP 1346)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1346.gb
(View with Genome Compiler) p1346.txt p1346.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Kanamycin (kan) |
| Replicon: | Escherichia coli plasmid p15A origin |
| Copy number: | Low copy number |
| Host range: | Escherichia coli |
| Parental clone: | p15A; Tn903; Tn3 |
| Further information: | This plasmid is compatible with ColE1- or pMB1-derived plasmids. In the presence of chloramphenicol or spectinomycin, the amplification of the plasmid is very limited (2x). The kanamycin resistance gene is excisable as a HaeII fragment. The nucleotide sequence of the vector corresponds with the EMBL Nucleotide Sequence Database accession number X06402.1, except for 2 nucleotides and a change in starting position. |
| EMBL Accession number: | X06402.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/01/1990 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AlwNI/HindIII and BamHI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr S. Cohen(1). (1) Stanford University School of Medicine, USA |
| Plasmid reference: | Chang et al., J. Bacteriol. 134 (1978), 1141-1156 [PMID: 149110] |
| Related plasmid reference: | Mok et al., Nucleic Acids Res. 19 (1991), 2321-2323 [PMID: 2041772] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | DSM 5587 NCCB 3009 PC-V 3009 |
Refer in your Materials and Methods: |
pACYC177 (LMBP 1346) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Cohen and was published in Chang et al., 1978. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.