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GeneCorner plasmid details

Last data update: 26 October 2020 04:25 CET

Plasmid name: pACYC177 (LMBP 1346)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p1346.gb (View with Genome Compiler)
p1346.txt
p1346.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Kanamycin (kan)
Replicon: Escherichia coli plasmid p15A origin
Host range: Escherichia coli
Parental clone: p15A; Tn903; Tn3
Further information: This plasmid is compatible with ColE1- or pMB1-derived plasmids.
In the presence of chloramphenicol or spectinomycin, the amplification of the plasmid is very limited (2x).
The kanamycin resistance gene is excisable as a HaeII fragment.
The nucleotide sequence of the vector corresponds with the EMBL Nucleotide Sequence Database accession number X06402.1, except for 2 nucleotides and a change in starting position.
EMBL Accession number: X06402.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 09/01/1990
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AlwNI/HindIII and BamHI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr S. Cohen(1).
(1) Stanford University School of Medicine, USA
Plasmid reference: Chang et al., J. Bacteriol. 134 (1978), 1141-1156 [PMID: 149110]
Related plasmid reference: Mok et al., Nucleic Acids Res. 19 (1991), 2321-2323 [PMID: 2041772]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: DSM 5587
NCCB 3009
PC-V 3009

Refer in your Materials and Methods:

pACYC177 (LMBP 1346) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Cohen and was published in Chang et al., 1978.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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