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GeneCorner plasmid details

Last data update: 20 October 2020 04:14 CEST

Plasmid name: pACYC184 (LMBP 992)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p992.gb (View with Genome Compiler)
p992.txt
p992.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Chloramphenicol (cam)
Tetracycline (tet)
Replicon: Escherichia coli plasmid p15A origin
Host range: Escherichia coli
Parental clone: p15A; pSC101; pKT002
Further information: This multicopy plasmid is compatible with ColE1- or pMB1-derived plasmids.
pACYC184 can be amplified 10-fold in the presence of spectinomycin.
The chloramphenicol resistance gene is excisable as a HaeII fragment.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number X06403.1.
EMBL Accession number: X06403.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 28/10/1994
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI/HindIII, EcoRI/XmnI, HindIII and RsaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr S. Cohen(1).
(1) Stanford University School of Medicine, USA
Plasmid reference: Chang et al., J. Bacteriol. 134 (1978), 1141-1156 [PMID: 149110]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 K514(λ)
Host reference: Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
Cultivation medium: LB-Lennox + chloramphenicol (25 μg/ml) + tetracycline (10 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with tetracycline.
Other culture collection numbers: DSM 4439
ATCC 37033
NCIB 12392
NRRL B-14221
NCCB 3011
PC-V 3010

Refer in your Materials and Methods:

pACYC184 (LMBP 992) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Cohen and was published in Chang et al., 1978.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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