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GeneCorner plasmid details

Last data update: 24 October 2020 04:12 CEST

Plasmid name: pACpLpA (LMBP 4236)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p4236.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human adenovirus 5 genome (Ad5); fragment
Promoter: -
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Human adenovirus 5 origin (Ad5)
Host range: Escherichia coli
Mammalian cells
Parental clone: pACCMVpLpA
Further information: The vector was constructed by deletion of the cytomegalovirus (CMV) early gene promoter/enhancer out of the pACCMVpLpA vector.
This plasmid is used in combination with e.g. pJM17 to generate recombinant Ad5 adenovirus.
Other name of the plasmid is pLpa-CMV.
EMBL Accession number: X02996, view at EMBL, GenBank, DDBJ
Latest sequence update: 01/08/2001
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AflIII, AflIII/EcoNI, AflIII/NotI, BamHI/SspI, BglI, EcoRI, HindII, NcoI, NdeI, NotI, SacI, SpeI and SspI.

The restriction site analysis pattern revealed that SspI is not unique, in contrast with the reconstructed nucleotide sequence.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr B. De Geest(1).
(1) Department of Transgene Technology and Gene Therapy, KUL, Leuven, Belgium
Plasmid reference: De Geest et al., Hum. Gene Ther. 11 (2000), 101-112 [PMID: 10646643]
Related plasmid reference: Becker et al., Methods Cell Biol. 43 (1994), 161-189 [PMID: 7823861]
Gomez-Foix et al., J. Biol. Chem. 267 (1992), 25129-25134 [PMID: 1334082]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pACpLpA (LMBP 4236) is available at BCCM/GeneCorner. This plasmid was deposited by Dr B. De Geest and was published in De Geest et al., 2000.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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