Last data update: 12 May 2021 19:50 CEST
Plasmid name: pAD140 (LMBP 9318)
|New search||Print data sheet|
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
Yersinia enterocolitica secretion protein gene D (yscD)|
Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP)
Bacillus subtilis levansucrase cDNA (sacB, GeneID 936413)
Escherichia coli insertion sequence IS1 insB
|Promoter:||Phage T7 gene 10 promoter (T7g10)|
|Selection marker:||Streptomycin (Sm)|
|Replicon:||Escherichia coli plasmid R6K origin; defective pir gene
Broad-host-range plasmid RK2/RP4 origin of transfer (oriT)
|Host range:||Escherichia coli; use strains supplying the R6K pir function
Gram-negative bacterial strains
|Parental clone:||pKNG101; pEGFP-C1|
|Further information:||The plasmid was constructed by cloning the EGFP CDS from pEGFP-C1 as an AgeI/EcoRI fragment into the flanking regions of Y. enterocolitica yscD and cloning the fused CDS as an ApaI/XbaI fragment into pKNG101.
This is a mutator plasmid to introduce the EGFP-YscD fusion protein.
Other name of the plasmid is pKNG101-EGFP-yscD.
|EMBL Accession number:||-|
|Latest sequence update:||03/07/2014|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr A. Diepold(2).
(1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium
(2) Biozentrum, University of Basel, Switzerland
|Plasmid reference:||Diepold et al., EMBO J. 29 (2010), 1928-1940 [PMID: 20453832]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 SM10(λpir)|
|Related host reference:||Simon et al., Biotechnology 1 (1983), 784-791
|Cultivation medium:||LB-Lennox + streptomycin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pAD140 (LMBP 9318) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Cornelis and was published in Diepold et al., 2010.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.