GeneCorner plasmid details

Last data update: 18 June 2021 07:57 CEST

Plasmid name: pAD4016 (LMBP 9321)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
analysis results


Cloned DNA: Yersinia enterocolitica arsenite pump transmembrane protein gene (arsB)
Yersinia enterocolitica arsenate reductase gene (arsC)
Yersinia enterocolitica arsenic resistance protein gene (arsH)
Yersinia enterocolitica arsenite inducible repressor gene (arsR)
Yersinia enterocolitica low calcium response protein genes (lcrD, lcrG, lcrR and lcrV)
Yersinia enterocolitica non-specific endonuclease gene (nuc)
Yersinia enterocolitica positive transcriptional regulator gene (pprA)
Yersinia enterocolitica plasmid pYV replication protein genes (repA and repB)
Yersinia enterocolitica plasmid pYV replication protein gene (repB)
Yersinia enterocolitica transcriptional regulator gene (spyA)
Yersinia enterocolitica DNA binding protein gene (spyB)
Yersinia enterocolitica specific yop chaperone gene (sycD, sycE, sycH, sycN and sycT)
Yersinia enterocolitica transposase gene (tnpA)
Yersinia enterocolitica resolvase gene (tnpR)
Yersinia enterocolitica DNA helicase gene (traI)
Yersinia enterocolitica IncF plasmid conjugative transfer pilin acetylase gene (traX)
Yersinia enterocolitica yop release control protein gene (tyeA)
Yersinia enterocolitica transcriptional activator gene (virF)
Yersinia enterocolitica lipoprotein gene (virG)
Yersinia enterocolitica outer membrane adhesin protein gene (yadA)
Yersinia enterocolitica surface exclusion protein gene (ylpA)
Yersinia enterocolitica integral membrane protein gene (yomA)
Yersinia enterocolitica anti-host protein genes (yopB, yopD, yopE, yopH, yopM, yopN, yopO, yopP, yopQ and yopT)
Yersinia enterocolitica secretion protein genes (yscA, yscB, yscC, yscD, yscE, yscF, yscG, yscH, yscI, yscJ, yscK, yscL, yscM1, yscM2, yscN, yscO, yscP, yscQ, yscR, yscS, yscT, yscU, yscV, yscW, yscX and yscY)
Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP)
Promoter: -
binding site:
Terminator: -
Selection marker: Arsenic (ars)
Replicon: Yersinia enterocolitica plasmid pYV40 ori
Host range: Yersinia enterocolitica
Parental clone: pYV40
Further information: The plasmid was constructed by allelic exchange of the wild type Y. enterocolitica yscQ gene of pYV40 and the EGFP-yscQ cassette of pAD118.
Other name of the plasmid is pYV40 egfp-yscQ+.
EMBL Accession number: -
Latest sequence update: 03/07/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr A. Diepold(2).
(1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium
(2) Biozentrum, University of Basel, Switzerland
Plasmid reference: Diepold et al., EMBO J. 29 (2010), 1928-1940 [PMID: 20453832]
Related plasmid reference: Sory et al., Proc. Natl. Acad. Sci. U.S.A. 92 (1995), 11998-12002 [PMID: 8618831]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Yersinia enterocolitica E40
Host reference: -
Cultivation medium: BHI + arsenic (400 μM) + nalidixic acid (35 μg/ml)
Cultivation temperature: 20°C
Biosafety level: L2
Cultivation remark: Cultivate on LB agar plates, or in liquid BHI.
Other culture collection numbers: -

Refer in your Materials and Methods:

pAD4016 (LMBP 9321) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Cornelis and was published in Diepold et al., 2010.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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