Last data update: 29 July 2021 10:25 CEST
Plasmid name: pAM1 (LMBP 8058)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p8058.gb
(View with Genome Compiler) p8058.txt p8058.pdf |
| Sequence analysis results Genecorner: |
|
| Cloned DNA: |
Bifidobacterium catenulatum L48 plasmid pBC1 replicase DNA (repB) Bifidobacterium catenulatum L48 plasmid pBC1 coatamer subunit gamma DNA (copG-like) Bifidobacterium catenulatum L48 plasmid pBC1 repB-transcriptionally coupled gene (orfX-like) |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Erythromycin (ery) |
| Replicon: | Escherichia coli plasmid pMB1 origin Bifidobacterium catenulatum L48 plasmid pBC1 origin |
| Host range: | Escherichia coli Bifidobacterium sp. |
| Parental clone: | pUC19E; pBC1 |
| Further information: | pAM1 is a shuttle vector that was constructed by ligating the BglII-opened pBC1 vector from B. catenulatum L48 into the BamHI-opened pUC19E vector. pUC19E consists of the pUC19 vector, with the erythromycin resistance gene of the Staphylococcus aureus plasmid pE194 ligated into its SmaI site. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726871.1. The nucleotide sequence of the pBC1 vector corresponds with the EMBL Nucleotide Sequence Database accession number DQ011664.1. The nucleotide sequence of pUC19 corresponds with the EMBL Nucleotide Sequence Database accession number M77789.2. The nucleotide sequence of the erythromycin insert corresponds with the EMBL Nucleotide Sequence Database accession number L08862.1. |
| EMBL Accession number: | DQ011664.1, view at EMBL, GenBank, DDBJ M77789.2, view at EMBL, GenBank, DDBJ L08862.1, view at EMBL, GenBank, DDBJ LT726871.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 10/12/2020 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI/XbaI, HaeII, HincII and HindIII. This plasmid has also been fully sequenced. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr B. Mayo(1). It was constructed by P. Alvarez-Martin(1). (1) IPLA-CSIC, Villaviciosa, Asturias, Spain |
| Plasmid reference: | Alvarez-Martin et al., Plasmid 57 (2007), 165-174 [PMID: 16930703] |
| Related plasmid reference: | Norrander et al., Gene 26 (1983), 101-106 [PMID: 6323249] Leenhouts et al., Appl. Environ. Microbiol. 57 (1991), 2562-2567 [PMID: 1768128] Alvarez-Martin et al., Appl. Environ. Microbiol. 74 (2008), 4656-4665 [PMID: 18539807] [DOI: 10.1128/AEM.00074-08] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | Cultivation medium for pAM1-carrying Bifidiobacterium strains: MRS + cysteine + erythromycin (5 μg/ml) or RCM + cysteine + erythromycin (5 μg/ml) |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pAM1 (LMBP 8058) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr B. Mayo and was published in Alvarez-Martin et al., 2007. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.