Last data update: 24 January 2024 16:39 CET
Plasmid name: pAM2005K (LMBP 3003)
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Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | - |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Erythromycin (ery) |
Replicon: | Enterococcus faecalis plasmid pAD1 origin |
Host range: | Enterococcus (formerly Streptococcus) faecalis Gram-positive bacterial strains |
Parental clone: | pAM2005E |
Further information: | pAM2005K is a miniplasmid from pAD1. The plasmid was derived from pAM2005E to reduce its size. It consists of the large KpnI fragment of pAM2005E, containing the Tn917-lacZ inducible fusion insert (NR5) and the traA, traB, C and E regions, shown to be important for pheromone regulation. The uvr gene encoding UV resistance is lost. |
EMBL Accession number: | - |
Latest sequence update: | 24/09/2001 |
Authenticity test: | By lack of sequence data, it was not possible to perform the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr D.B. Clewell(1). (1) University of Michigan, USA |
Plasmid reference: | Weaver et al., Plasmid 22 (1989), 106-119 [PMID: 2516332] |
Related plasmid reference: | Weaver et al., J. Bacteriol. 172 (1990), 2633-2641 [PMID: 2158976] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Enterococcus faecalis OG1X |
Host reference: | - |
Cultivation medium: | MRS + erythromycin (5 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L2 |
Cultivation remark: | The Enterococcus faecalis OG1X strain bears also resistance to streptomycin. |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pAM2005K (LMBP 3003) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D.B. Clewell and was published in Weaver et al., 1989. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.