Last data update: 21 October 2020 04:26 CEST
Plasmid name: pAM2005K (LMBP 3003)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Depositor's sequence:||not available|
|Selection marker:||Erythromycin (ery)|
|Replicon:||Enterococcus faecalis plasmid pAD1 origin|
|Host range:||Enterococcus (formerly Streptococcus) faecalis
Gram-positive bacterial strains
|Further information:||pAM2005K is a miniplasmid from pAD1.
The plasmid was derived from pAM2005E to reduce its size. It consists of the large KpnI fragment of pAM2005E, containing the Tn917-lacZ inducible fusion insert (NR5) and the traA, traB, C and E regions, shown to be important for pheromone regulation. The uvr gene encoding UV resistance is lost.
|EMBL Accession number:||-|
|Latest sequence update:||24/09/2001|
|Authenticity test:||By lack of sequence data, it was not possible to perform the authenticity test.|
|History of deposit:||This plasmid was deposited by Dr D.B. Clewell(1).
(1) University of Michigan, USA
|Plasmid reference:||Weaver et al., Plasmid 22 (1989), 106-119 [PMID: 2516332]
|Related plasmid reference:||Weaver et al., J. Bacteriol. 172 (1990), 2633-2641 [PMID: 2158976]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Enterococcus faecalis OG1X|
|Cultivation medium:||MRS + erythromycin (5 μg/ml)|
|Cultivation remark:||The Enterococcus faecalis OG1X strain bears also resistance to streptomycin.|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pAM2005K (LMBP 3003) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D.B. Clewell and was published in Weaver et al., 1989.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.