GeneCorner plasmid details

Last data update: 17 September 2021 10:55 CEST

Plasmid name: pAM2005K (LMBP 3003)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
analysis results


Cloned DNA: -
Promoter: -
binding site:
Terminator: -
Selection marker: Erythromycin (ery)
Replicon: Enterococcus faecalis plasmid pAD1 origin
Host range: Enterococcus (formerly Streptococcus) faecalis
Gram-positive bacterial strains
Parental clone: pAM2005E
Further information: pAM2005K is a miniplasmid from pAD1.
The plasmid was derived from pAM2005E to reduce its size. It consists of the large KpnI fragment of pAM2005E, containing the Tn917-lacZ inducible fusion insert (NR5) and the traA, traB, C and E regions, shown to be important for pheromone regulation. The uvr gene encoding UV resistance is lost.
EMBL Accession number: -
Latest sequence update: 24/09/2001
Authenticity test: By lack of sequence data, it was not possible to perform the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr D.B. Clewell(1).
(1) University of Michigan, USA
Plasmid reference: Weaver et al., Plasmid 22 (1989), 106-119 [PMID: 2516332]
Related plasmid reference: Weaver et al., J. Bacteriol. 172 (1990), 2633-2641 [PMID: 2158976]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Enterococcus faecalis OG1X
Host reference: -
Cultivation medium: MRS + erythromycin (5 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L2
Cultivation remark: The Enterococcus faecalis OG1X strain bears also resistance to streptomycin.
Other culture collection numbers: -

Refer in your Materials and Methods:

pAM2005K (LMBP 3003) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D.B. Clewell and was published in Weaver et al., 1989.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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