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GeneCorner plasmid details

Last data update: 21 October 2020 04:26 CEST

Plasmid name: pAOX2BAX (LMBP 6634)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p6634.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse Bcl2-associated X cDNA (Bax, BAX); α variant
Promoter: Pichia pastoris alcohol oxidase 2 promoter (AOX2)
Ribosome
binding site:
-
Terminator: Pichia pastoris alcohol oxidase 1 terminator (AOX1)
Selection marker: Ampicillin (amp)
Pichia pastoris HIS4; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Pichia pastoris; his4(-), integrative
Parental clone: pUC19B; pAOX2
Further information: The plasmid was created by isolating the mBax cDNA from pUC19B as a HindIII (Klenow blunted)/EcoRI fragment and cloning it into the XhoI (Klenow blunted)/EcoRI-opened pAOX2 vector.
The nucleotide sequence of the mBax cDNA corresponds with the EMBL Nucleotide Sequence Database accession number L22472.1.
EMBL Accession number: L22472.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 16/02/2010
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI, EcoRV, EcoRV/SphI, SacI, SacI/SacII, SacII, SphI and XmnI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr. P. Tiels(1) (2) and Prof. Dr N. Callewaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
Plasmid reference: Martinet et al., Biotechnol. Lett. 21 (1999), 821-829
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pAOX2BAX (LMBP 6634) is available at BCCM/GeneCorner. This plasmid was deposited by Dr. P. Tiels and Prof. Dr N. Callewaert and was published in Martinet et al., 1999.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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