Last data update: 24 January 2024 16:39 CET
Plasmid name: pAOX2BAX (LMBP 6634)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6634.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse Bcl2-associated X cDNA (Bax, BAX); α variant |
| Promoter: | Pichia pastoris alcohol oxidase 2 promoter (AOX2) |
| Ribosome binding site: |
- |
| Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) |
| Selection marker: | Ampicillin (amp) Pichia pastoris HIS4; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Pichia pastoris; his4(-), integrative |
| Parental clone: | pUC19B; pAOX2 |
| Further information: | The plasmid was created by isolating the mBax cDNA from pUC19B as a HindIII (Klenow blunted)/EcoRI fragment and cloning it into the XhoI (Klenow blunted)/EcoRI-opened pAOX2 vector. The nucleotide sequence of the mBax cDNA corresponds with the EMBL Nucleotide Sequence Database accession number L22472.1. |
| EMBL Accession number: | L22472.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 16/02/2010 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI, EcoRV, EcoRV/SphI, SacI, SacI/SacII, SacII, SphI and XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr. P. Tiels(1) (2) and Prof. Dr N. Callewaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Martinet et al., Biotechnol. Lett. 21 (1999), 821-829 |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pAOX2BAX (LMBP 6634) is available at BCCM/GeneCorner. This plasmid was deposited by Dr. P. Tiels and Prof. Dr N. Callewaert and was published in Martinet et al., 1999. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.