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GeneCorner plasmid details

Last data update: 30 November 2020 04:30 CET

Plasmid name: pAOX2ZB (LMBP 5799)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p5799.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Pichia pastoris alcohol oxidase 2 promoter (AOX2)
Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α)
Synthetic prokaryotic EM7 promoter
Ribosome
binding site:
-
Terminator: Pichia pastoris alcohol oxidase 1 terminator (AOX1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Selection marker: Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo))
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; preferably recombination-deficient and endonuclease A deficient strains
Pichia pastoris; integrative
Parental clone: pPICZB
Further information: The plasmid was created by PCR amplifying the Pichia AOX2 promotor sequence and cloning it as an EcoRI-BglII fragment into the BglII-EcoRI opened pPICZB vector.
EMBL Accession number: -
Latest sequence update: 17/03/2009
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr N. Callewaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
Plasmid reference: Martinet et al., Biotechnol. Lett. 21 (1999), 821-829
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + zeocin (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pAOX2ZB (LMBP 5799) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert and was published in Martinet et al., 1999.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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