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GeneCorner plasmid details

Last data update: 30 October 2020 04:11 CET

Plasmid name: pAS104 (LMBP 4356)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4356.gb (View with Genome Compiler)
p4356.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: E35S promoter/plant junction region; 359 bp part of the synthetic construct
Promoter: Escherichia coli lac operon promoter
Phage SP6 promoter
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Neomycin (neo; kanamycin (kan))
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pCRII
Further information: The plasmid was constructed by ligating a 359 bp fragment of the E35S promoter/plant junction region into the pCRII vector with the TA cloning Kit Dual Promoter (Invitrogen).
The cloned DNA consists of a junction between part of the 35S promoter from the Cauliflower Mosaic virus (CaMV) and plant DNA, as present in the transgenic Roundup Ready (RR) soybean line event 40-3-2 (Monsanto). The CaMV promoter fragment is used as a promoter gene in this transgenic event.
The E35S promoter/plant junction is highly specific for RR soybean and admits unambiguous identification of the genetically modified organism.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727196.1.
The nucleotide sequence of the inserted E35S promoter/plant junction region fragment corresponds with the EMBL Nucleotide Sequence Database (Release 67; Accession number AJ308514).
EMBL Accession number: AJ308514, view at EMBL, GenBank, DDBJ
LT727196.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 15/06/2001
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI, EcoRV and HindIII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by I. Taverniers(1).
(1) Department Plant Genetics and Breeding, CLO-Gent, Belgium
Plasmid reference: Taverniers et al., Eur. Food Res. Technol. 213 (2001), 417-424
Related plasmid reference: Van den Bulcke et al. (2006)
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 INVαF'
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pAS104 (LMBP 4356) is available at BCCM/GeneCorner. This plasmid was deposited by I. Taverniers and was published in Taverniers et al., 2001.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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