Last data update: 30 October 2020 04:11 CET
Plasmid name: pAS104 (LMBP 4356)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||E35S promoter/plant junction region; 359 bp part of the synthetic construct
|Promoter:||Escherichia coli lac operon promoter
Phage SP6 promoter
Phage T7 gene 10 promoter (T7g10)
|Selection marker:||Ampicillin (amp)
Neomycin (neo; kanamycin (kan))
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli|
|Further information:||The plasmid was constructed by ligating a 359 bp fragment of the E35S promoter/plant junction region into the pCRII vector with the TA cloning Kit Dual Promoter (Invitrogen).
The cloned DNA consists of a junction between part of the 35S promoter from the Cauliflower Mosaic virus (CaMV) and plant DNA, as present in the transgenic Roundup Ready (RR) soybean line event 40-3-2 (Monsanto). The CaMV promoter fragment is used as a promoter gene in this transgenic event.
The E35S promoter/plant junction is highly specific for RR soybean and admits unambiguous identification of the genetically modified organism.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727196.1.
The nucleotide sequence of the inserted E35S promoter/plant junction region fragment corresponds with the EMBL Nucleotide Sequence Database (Release 67; Accession number AJ308514).
|EMBL Accession number:||AJ308514, view at EMBL, GenBank, DDBJ
LT727196.1, view at EMBL, GenBank, DDBJ
|Latest sequence update:||15/06/2001|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: EcoRI, EcoRV and HindIII.|
|History of deposit:||This plasmid was deposited by I. Taverniers(1).
(1) Department Plant Genetics and Breeding, CLO-Gent, Belgium
|Plasmid reference:||Taverniers et al., Eur. Food Res. Technol. 213 (2001), 417-424
|Related plasmid reference:||Van den Bulcke et al. (2006)
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 INVαF'|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pAS104 (LMBP 4356) is available at BCCM/GeneCorner. This plasmid was deposited by I. Taverniers and was published in Taverniers et al., 2001.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.