Last data update: 24 January 2024 16:39 CET
Plasmid name: pAS104 (LMBP 4356)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4356.gb
(View with Genome Compiler) p4356.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
E35S promoter/plant junction region; 359 bp part of the synthetic construct |
| Promoter: | Escherichia coli lac operon promoter Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pCRII |
| Further information: | The plasmid was constructed by ligating a 359 bp fragment of the E35S promoter/plant junction region into the pCRII vector with the TA cloning Kit Dual Promoter (Invitrogen). The cloned DNA consists of a junction between part of the 35S promoter from the Cauliflower Mosaic virus (CaMV) and plant DNA, as present in the transgenic Roundup Ready (RR) soybean line event 40-3-2 (Monsanto). The CaMV promoter fragment is used as a promoter gene in this transgenic event. The E35S promoter/plant junction is highly specific for RR soybean and admits unambiguous identification of the genetically modified organism. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727196.1. The nucleotide sequence of the inserted E35S promoter/plant junction region fragment corresponds with the EMBL Nucleotide Sequence Database (Release 67; Accession number AJ308514). |
| EMBL Accession number: | AJ308514, view at EMBL, GenBank, DDBJ LT727196.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 15/06/2001 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, EcoRV and HindIII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by I. Taverniers(1). (1) Department Plant Genetics and Breeding, CLO-Gent, Belgium |
| Plasmid reference: | Taverniers et al., Eur. Food Res. Technol. 213 (2001), 417-424 [DOI: 10.1007/s002170100405] |
| Related plasmid reference: | Van den Bulcke et al. (2006) Windels et al., Med. Fac. Landbouww. Univ. Gent 64/5b (1999), 459-462 Windels et al., Eur. Food Res. Technol. 213 (2001), 107-112 |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 INVαF' |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pAS104 (LMBP 4356) is available at BCCM/GeneCorner. This plasmid was deposited by I. Taverniers and was published in Taverniers et al., 2001. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.