Last data update: 24 January 2024 16:39 CET
Plasmid name: pAS106 (LMBP 4357)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p4357.gb
(View with Genome Compiler) p4357.txt p4357.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Soybean lectin gene (Le1); 118 bp part of the mature sequence |
Promoter: | Escherichia coli lac operon promoter Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) Neomycin (neo; kanamycin (kan)) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
Host range: | Escherichia coli |
Parental clone: | pCRII |
Further information: | The plasmid was constructed by ligating a 118 bp part of the mature Le1 sequence into the pCRII vector with the TA cloning Kit Dual Promoter (Invitrogen). When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. Taverniers et al. (2001) is online searchable via DOI 10.1007/s002170100405, resulting in the following web site: http://www.springerlink.com/content/w7e7j7c0agy07058/. The nucleotide sequence of the Le1 fragment corresponds with the EMBL Nucleotide Sequence Database accession number K00821.1. |
EMBL Accession number: | K00821.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 15/06/2001 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindIII and PstI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by I. Taverniers(1). (1) Department for Plant Genetics and Breeding, CLO-Gent, Belgium |
Plasmid reference: | Taverniers et al., Eur. Food Res. Technol. 213 (2001), 417-424 [DOI: 10.1007/s002170100405] |
Related plasmid reference: | Van den Bulcke et al. (2006) |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 INVαF' |
Host reference: | - |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pAS106 (LMBP 4357) is available at BCCM/GeneCorner. This plasmid was deposited by I. Taverniers and was published in Taverniers et al., 2001. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.