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GeneCorner plasmid details

Last data update: 29 October 2020 04:15 CET

Plasmid name: pAS2-IKKepsilon (LMBP 6916)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p6916.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human inhibitor of nuclear factor kappa B kinase subunit epsilon cDNA (IKBKE, IKK-i, IKKE, GeneID 9641)
Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd)
Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal
Promoter: Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator
Selection marker: Ampicillin (amp)
Cycloheximide (CYH2)
TRP1; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB)
Host range: Escherichia coli
Saccharomyces cerevisiae; trp1(-)
Parental clone: pAS2; pcDNA3.1-FLAG-IKKe
Further information: The plasmid was constructed via PCR amplification of IKKepsilon with pcDNA3.1-FLAG-IKKe as a template. The IKKe cds was ligated as an NcoI/BamHI fragment into the NcoI/BamHI opened pAS2 vector.
The nucleotide sequence of the pAS2 vector corresponds with the EMBL Nucleotide Sequence Database accession number U30496.1.
The nucleotide sequence of the IKKe cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF241789.1, except for a G202A nucleotide substitution, leading to a V68I amino acid mutation, and a silent C1350A nucleotide substitution.
EMBL Accession number: U30496.1, view at EMBL, GenBank, DDBJ
AF241789.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/04/2015
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by K. Verhelst(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pAS2-IKKepsilon (LMBP 6916) is available at BCCM/GeneCorner. This plasmid was deposited by K. Verhelst and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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