Last data update: 24 January 2024 16:39 CET
Plasmid name: pAS2-IKKgammaC-MUT2 (LMBP 5504)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human inhibitor of nuclear factor kappa B kinase regulatory subunit gamma cDNA (IKBKG, IP2, IP1, IKK-gamma, NEMO, FIP-3, FIP3, ZC2HC9, IKKG, GeneID 8517); mutated ABIN Homology Domain 2 (AHD2) Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal |
Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Escherichia coli lac operon promoter |
Ribosome binding site: |
- |
Terminator: | Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator |
Selection marker: | Ampicillin (amp) Cycloheximide (CYH2) TRP1; auxotrophic |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
Host range: | Escherichia coli Saccharomyces cerevisiae; trp1(-) |
Parental clone: | pAS2; pAS2-IKKgamma-MUT2 |
Further information: | The plasmid was constructed by inserting the MscI/AlwNI fragment of pAS2-IKKgamma-MUT2, containing the mutated human IKKγ C-terminal fragment, into the MscI-AlwNI opened pAS2 vector. As a result, the inserted DNA is fused in phase to the N-terminal HA epitope and the S. cerevisiae GALbd domain. Yeast two hybrid The plasmid contains the C-terminal fragment of hIKKγ (AA275-419) with mutation in AHD2 (DF -> NA). Other name of the plasmid is pAS2-IKKγC-MUT2. |
EMBL Accession number: | AJ271718, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 03/03/2008 |
Sequence detail: | Amino acid sequece of human IKKgamma: IKKgamma wild type: 300 VLKAQADIYKADFQAERQAREKL 322 IKKgamma MUT1 : 300 VLKEEADIYKADFQAERQAREKL 322 ** IKKgamma MUT2 : 300 VLKAQADIYKANAQAERQAREKL 322 ** IKKgamma MUT3 : 300 VLKAQADIYKADFQAERQAAQKL 322 ** *: mutated amino acid |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr K. Heyninck(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Related plasmid reference: | Heyninck et al., FEBS Lett. 536 (2003), 135-140 [PMID: 12586352] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pAS2-IKKgammaC-MUT2 (LMBP 5504) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. Heyninck and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.