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GeneCorner plasmid details

Last data update: 20 October 2020 04:14 CEST

Plasmid name: pAS2-mMyD88ID (LMBP 5654)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse myeloid differentiation primary response gene 88 cDNA (Myd88, GeneID 17874); intermediate domain Myd88ID (aa 110-157)
Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd)
Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal
Promoter: Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator
Selection marker: Ampicillin (amp)
Cycloheximide (CYH2)
TRP1; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB)
Host range: Escherichia coli
Saccharomyces cerevisiae; trp1(-)
Parental clone: pCAGGS-E-mMyD88
Further information: Yeast 2-Hybrid construction.
The mouse myeloid differentiation primary response gene 88 cDNA (Myd88) encodes an adaptor protein involved in TLR and IL-1R induced activation of NF-κB.
Mouse Myd88ID consists of exon 2, the intermediate domain (aa 110-154).
Mouse Myd88ID was picked up and cloned in frame with the N-terminal GALbd.
The nucleotide sequence of the mouse Myd88 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number U84409.1.
EMBL Accession number: U84409.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 25/01/2008
Sequence detail:
110 111 112 113 114  
Forward primer: 5' GGA.ATC.CCG.GGC.ATG.GAG.GAG.GAC.TCG.CAG 3'
                         SmaI
                         XmaI

                                        154 153 152 151 150
Reverse primer: 5' CGCCCTGCAGCTCGAG.TCA.TAG.GGG.GTC.ATC.AAG 3'
                        PstI
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr S. Janssens(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Janssens et al., Curr. Biol. 12 (2002), 467-471 [PMID: 11909531]
Janssens et al., Trends Biochem. Sci. 27 (2002), 474-482 [PMID: 12217523]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pAS2-mMyD88ID (LMBP 5654) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Janssens and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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