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GeneCorner plasmid details

Last data update: 23 October 2020 04:27 CEST

Plasmid name: pAT153 (LMBP 735)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: pat153.gb (View with Genome Compiler)
pat153.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322
Further information: This plasmid was derived from pBR322 by deletion of a 622 bp Haell (nucleotide positions 1727 and 2347) fragment.
pAT153 is a pBR322 derivative in which the bom site as well as the region coding for the rop protein have been deleted. The plasmid can not be mobilized. It displays a 2 to 3 fold higher copy number than pBR322.
The HindIII site does not always inactivate the tetracycline gene.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726857.1.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database (Release 37; Accession number L08853).
EMBL Accession number: L08853, view at EMBL, GenBank, DDBJ
LT726857.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 23/01/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: HindII and PstI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr D. Sherratt(1).
(1) University of Glasgow, Scotland
Plasmid reference: Twigg et al., Nature 283 (1980), 216-218 [PMID: 7350544]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: DSM 3864
NCIB 12206
NCTC 50242
NCCB 3011
PC-V 3011

Refer in your Materials and Methods:

pAT153 (LMBP 735) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D. Sherratt and was published in Twigg et al., 1980.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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