Last data update: 21 January 2021 04:16 CET
Plasmid name: pAT153 (LMBP 735)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||This plasmid was derived from pBR322 by deletion of a 622 bp Haell (nucleotide positions 1727 and 2347) fragment.
pAT153 is a pBR322 derivative in which the bom site as well as the region coding for the rop protein have been deleted. The plasmid can not be mobilized. It displays a 2 to 3 fold higher copy number than pBR322.
The HindIII site does not always inactivate the tetracycline gene.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726857.1.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database (Release 37; Accession number L08853).
|EMBL Accession number:||L08853, view at EMBL, GenBank, DDBJ
LT726857.1, view at EMBL, GenBank, DDBJ
|Latest sequence update:||23/01/1989|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: HindII and PstI.|
|History of deposit:||This plasmid was deposited by Dr D. Sherratt(1).
(1) University of Glasgow, Scotland
|Plasmid reference:||Twigg et al., Nature 283 (1980), 216-218 [PMID: 7350544]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)|
|Other culture collection numbers:||DSM 3864
Refer in your Materials and Methods:
|pAT153 (LMBP 735) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D. Sherratt and was published in Twigg et al., 1980.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.