Last data update: 28 October 2020 04:17 CET
Plasmid name: pAT153HIL2 (LMBP 1215)
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1215.gb
(View with Genome Compiler) p1215.txt p1215.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | Human interleukin 2 cDNA (IL2, IL-2, TCGF, GeneID 3558); mature sequence |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pAT153; pSV-HIL2-0 |
| Further information: | The human interleukin 2 (hIL2) fragment in the plasmid is the cDNA fragment derived from mRNA isolated from human splenocytes, and cloned in the BamHI site of pAT153 via dC/dG-tailing. It is not clear whether the hIL2 sequence starts with the last or the second last codon of the signal peptide. This plasmid contains the entire mature region of the hIL2 gene as well as the total 3' end of the mRNA. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727571.1. The nucleotide sequence of the human IL2 corresponds with the EMBL Nucleotide Sequence Database accession number X01586.1. |
| EMBL Accession number: | X01586.1, view at EMBL, GenBank, DDBJ LT727571.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 26/04/1994 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BglI, Eco130I and XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Devos et al., Nucleic Acids Res. 11 (1983), 4307-4323 [PMID: 6306584] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
|
Refer in your Materials and Methods: |
pAT153HIL2 (LMBP 1215) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Devos et al., 1983. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.