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GeneCorner plasmid details

Last data update: 27 October 2020 04:27 CET

Plasmid name: pAT153MIL2F2 (LMBP 4731)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4731.gb (View with Genome Compiler)
p4731.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse interleukin 2 cDNA (IL2)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pAT153
Further information: This plasmid contains the mouse interleukin 2 cDNA (mIL2) from the mouse mRNA EL4 ExC5 cell line, cloned via dG/dC-tailing in the unique PstI site of pAT153.
The length of the 5' and 3' untranslated regions of the mIL2 insert is not exactly known.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727177.1.
The (+-) 900 bp mIL2 insert has not been entirely sequenced (only the PstI site in front of the mIL2 start codon).
The nucleotide sequence of the mouse IL2 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number X01772.1.
Other name of the plasmid is 14-19-2F.
EMBL Accession number: X01772.1, view at EMBL, GenBank, DDBJ
LT727177.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 14/07/2003
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI/HindIII, BglI, EcoRI, PstI and SacI/XmnI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by J. Demolder(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: LMBP 01352

Refer in your Materials and Methods:

pAT153MIL2F2 (LMBP 4731) is available at BCCM/GeneCorner. This plasmid was deposited by J. Demolder and Prof. Dr E. Remaut.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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