Last data update: 20 October 2020 04:14 CEST
Plasmid name: pAT153cI857 (LMBP 1065)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Phage λ cI temperature-sensitive repressor gene (cIts, cI857)
|Promoter:||Phage λ major rightward promoter (λ PR)
Phage λ promoter for repressor maintenance (λ PRM)
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pAT153; pBR322cI857; pPR395Ec|
|Further information:||Designed for the expression of fragments containing a ribosome binding site functional in E. coli.
The λ PR promoter is controlled by the cI857 temperature sensitive repressor that is cloned on the same plasmid.
The length of the dG/dC-tail behind the cI857 gene is not known.
Other name of the plasmid is pAT153cI6.
|EMBL Accession number:||-|
|Latest sequence update:||25/03/2011|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||PhD thesis Eugeen Vanmechelen (1986)
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pAT153cI857 (LMBP 1065) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Eugeen Vanmechelen, 1986.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.