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GeneCorner plasmid details

Last data update: 20 October 2020 04:14 CEST

Plasmid name: pATE6Hy3 (LMBP 1677)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pate6hy3.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) half genomic gene; mouse::human chimeric heavy chain (E6Hy3)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pAT153; pSV23SE6H; pATHIG32
Further information: The E6Hy3 insert is a fusion between the HindIII-HinFI fragment of pSV23SE6H, encoding L, VH and 2/3 of CH1 of a mouse IgG2b heavy chain, and the HinFI-SalI fragment of pATHIG32, encoding the last 37 amino acids of CH1, the complete hinge region, CH2 and CH3 of the human γ3 heavy chain.
The E6Hy3 insert encodes the complete mouse: human chimeric heavy chain of the anti-tumor antibody E6(Hy3,κ).
The chimeric CH1 domain contains 60 amino acids of mouse (E6H) and 37 amino acids of human (hIG3) origin.
The mouse part of this vector consists of cDNA; the human part consists of genomic DNA.
Other name of the plasmid is pATHy3.
EMBL Accession number: -
Latest sequence update: 05/04/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: De Sutter et al., Gene 113 (1992), 223-230 [PMID: 1572543]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB HB101
Host reference: Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
Related host reference: Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pATE6Hy3 (LMBP 1677) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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