Last data update: 24 January 2024 16:39 CET
Plasmid name: pATHIFNBg43 (LMBP 1656)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1656.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interferon β gene (IFNB, IFNB1) |
| Promoter: | Human interferon β promoter (IFNB) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pATHIFNBg42 |
| Further information: | The plasmid is derived from pATHIFNBg42 by deletion of a HindII-BamHI fragment, the 3' untranslated region of the hIFNβ gene remains intact up to the polyA signal. See also pATHIFNBg42 for basic constructions. The plasmid contains the genomic hIFNβ fragment with 3' and 5' flanking ends. Other names of the plasmid are pg4-delB-delHB and pAT153gHIFNB3. |
| EMBL Accession number: | - |
| Latest sequence update: | 17/09/1987 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by W. Kempenaers(1) and Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Walter Kempenaers (1985) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pATHIFNBg43 (LMBP 1656) is available at BCCM/GeneCorner. This plasmid was deposited by W. Kempenaers and Prof. Dr E. Remaut and was published in Walter Kempenaers, 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.