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GeneCorner plasmid details

Last data update: 24 October 2020 04:12 CEST

Plasmid name: pATHIFNBg43 (LMBP 1656)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1656.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interferon β gene (IFNB, IFNB1)
Promoter: Human interferon β promoter (IFNB)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pATHIFNBg42
Further information: The plasmid is derived from pATHIFNBg42 by deletion of a HindII-BamHI fragment, the 3' untranslated region of the hIFNβ gene remains intact up to the polyA signal. See also pATHIFNBg42 for basic constructions.
The plasmid contains the genomic hIFNβ fragment with 3' and 5' flanking ends.
Other names of the plasmid are pg4-delB-delHB and pAT153gHIFNB3.
EMBL Accession number: -
Latest sequence update: 17/09/1987
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by W. Kempenaers(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: PhD thesis Walter Kempenaers (1985)
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pATHIFNBg43 (LMBP 1656) is available at BCCM/GeneCorner. This plasmid was deposited by W. Kempenaers and Prof. Dr E. Remaut and was published in Walter Kempenaers, 1985.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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