Last data update: 29 October 2020 04:15 CET
Plasmid name: pATHIG31 (LMBP 1674)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human immunoglobulin γ3 genomic DNA (hIG3); fragment of the constant portion of the heavy chain
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||Derived from pAT153 by inserting the human immunoglobulin γ3 gene as an EcoRI-HindIII fragment. This insert, which was isolated from the partial HaeIII-AluI Charon 4A genomic library (Maniatis), encodes the constant portion of the human γ3 heavy chain only.
In B-cell differentiation, the 5' flanking region of the hIG3 gene is removed by joining to the J region.
Since part of the nucleotide sequence of the 3' flanking region of the hIG3 insert (between nucleotide position 4 and 1113 in pATHIG31) is unknown, there is uncertainty as to the cutting frequency of the restriction enzymes indicated.
The nucleotide sequence of the hIG3 sequence used for the computer enzyme map is taken from Huck et al. (1986) and some differences with our cloned hIG3 gene can be expected: e.g. 1) at nucleotide position 966 of hIG3 we found a C while an A is mentioned in Huck et al.; 2) at nucleotide position 1158 we found a G instead of the A mentioned by Huck et al. (1986).
Other name of the plasmid is pATHuIG31.
|EMBL Accession number:||-|
|Latest sequence update:||05/04/1989|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Related plasmid reference:||Huck et al., Nucleic Acids Res. 4 (1986), 1779-1789 [PMID: 3081877]
Krawinkel et al., EMBO J. 1 (1982), 403-407 [PMID: 6329676]
Takahashi et al., Cell 29 (1982), 671-679 [PMID: 6811139]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12xB HB101|
|Host reference:||Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
|Related host reference:||Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pATHIG31 (LMBP 1674) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.