Last data update: 26 October 2020 04:25 CET
Plasmid name: pATHIG4 (LMBP 1675)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human immunoglobulin γ4 genomic DNA (hIG4); fragment of the constant portion of the heavy chain
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||The hIG4 gene was isolated from the partial HaeIII-AluI human genomic library (Maniatis) and subsequently cloned as a HindIII-EcoRI fragment of approx. 2000 nucleotides into pAT153.
The possible expression of the tetracycline resistance gene was not tested.
The sequence from base position 4 to approx. 13 is unknown !!!
U-BalI only cuts in dcm negative strains !!!
The nucleotide sequence of hIG4 used for the computer map is taken from the publication of Ellison et.al. (1981).
Other name of the plasmid is pATHuIG4 !!
|EMBL Accession number:||-|
|Latest sequence update:||20/11/1987|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Related plasmid reference:||Ellison et al., DNA 1 (1981), 11-18 [PMID: 6299662]
Krawinkel et al., EMBO J. 1 (1982), 403-407 [PMID: 6329676]
Takahashi et al., Cell 29 (1982), 671-679 [PMID: 6811139]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12xB HB101|
|Host reference:||Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
|Related host reference:||Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pATHIG4 (LMBP 1675) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.