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GeneCorner plasmid details

Last data update: 20 October 2020 04:14 CEST

Plasmid name: pATHIL6RD3R (LMBP 2680)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2680.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Rat dopamine D3 receptor (rD3R)
Promoter: Human interleukin 6 promoter (IL6)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pATHIL6pA; pUC18RD3R
Further information: This plasmid was constructed as follows: pUC18RD3R was opened with EcoRI and filled-in with Klenow DNA polymerase. After digestion with XbaI the dopamine receptor (rD3R) was isolated and ligated into the StuI-XbaI opened pATHIL6pA. In this way the rat dopamine D3 receptor became under control of the hIL6 promoter.
The nucleotide sequence of the genomic human IL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11, except for the C nucleotide at promoter position -174 (polymorphism mutant) which was changed into a G nucleotide (wild type).
Other name of the plasmid is pATHIL6PrDop3R.
EMBL Accession number: AC073072.11, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/05/2004
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pATHIL6RD3R (LMBP 2680) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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