Last data update: 20 October 2020 04:14 CEST
Plasmid name: pATHIL6RD3R (LMBP 2680)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Rat dopamine D3 receptor (rD3R)
|Promoter:||Human interleukin 6 promoter (IL6)|
|Terminator:||Simian virus 40 polyadenylation signal (SV40 polyA)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pATHIL6pA; pUC18RD3R|
|Further information:||This plasmid was constructed as follows: pUC18RD3R was opened with EcoRI and filled-in with Klenow DNA polymerase. After digestion with XbaI the dopamine receptor (rD3R) was isolated and ligated into the StuI-XbaI opened pATHIL6pA. In this way the rat dopamine D3 receptor became under control of the hIL6 promoter.
The nucleotide sequence of the genomic human IL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11, except for the C nucleotide at promoter position -174 (polymorphism mutant) which was changed into a G nucleotide (wild type).
Other name of the plasmid is pATHIL6PrDop3R.
|EMBL Accession number:||AC073072.11, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||03/05/2004|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pATHIL6RD3R (LMBP 2680) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.