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GeneCorner plasmid details

Last data update: 03 December 2020 04:26 CET

Plasmid name: pATHIL6pA (LMBP 2659)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p2659.gb (View with Genome Compiler)
p2659.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Human interleukin 6 promoter (IL6)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pMaSVTHIL61; pSV23S; pAT153
Further information: The plasmid was constructed by a three fragment ligation: 1) the EcoRI-StuI fragment of pMaSVTHIL61, containing the human IL6 promoter; 2) the StuI-BamHI fragment of pSV23S, containing the SV40 polyA and the SV40 small t-antigen splicing signal; 3) the BamHI-EcoRI opened pAT153 vector. In this way, 5 unique sites (AvrII, HindIII, StuI, XbaI, XhoI) were obtained to clone a gene under control of the human IL6 promoter.
The tetracycline resistance gene was disrupted.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727532.1.
The nucleotide sequence of the genomic human IL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11, except for the C nucleotide at promoter position -174 (polymorphism mutant) which was changed into a G nucleotide (wild type).
Other name of the plasmid is pATIL6PpolyA.
EMBL Accession number: AC073072.11, view at EMBL, GenBank, DDBJ
LT727532.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/05/2004
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI/StuI, EcoRI/PstI, HindII, PvuI/XhoI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. Vanhoenacker(1) and Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pATHIL6pA (LMBP 2659) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Vanhoenacker and Prof. Dr G. Haegeman.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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