GeneCorner plasmid details

Last data update: 30 July 2021 10:22 CEST

Plasmid name: pATHTNF (LMBP 2529)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: (View with Genome Compiler)
analysis results


Cloned DNA: Human tumor necrosis factor cDNA (TNF)
Promoter: -
binding site:
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pAT153
Further information: This plasmid was constructed as follows: dG/dC-tailed, human TNF cDNA was inserted into the filled-in PstI site of pAT153. The orientation of the inserted fragment was confirmed by an EcoRI digestion.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727524.1.
The nucleotide sequence of the human TNF cDNA corresponds with the EMBL Nucleotide Sequence Database accession number X01394.1.
Name mentioned in Marmenout et al. (1985) is phTNF1.
EMBL Accession number: X01394.1, view at EMBL, GenBank, DDBJ
LT727524.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 04/01/1991
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, Eco47III, EcoRI, HincII/RsaI and PstI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr W. Fiers(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Marmenout et al., Eur. J. Biochem. 152 (1985), 515-522 [PMID: 3932069]
Related plasmid reference: Söderberg et al. 43 (2007), 90-99 [PMID: 17561097] [DOI: 10.1016/j.freeradbiomed.2007.03.026]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH1(λ)
Host reference: Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pATHTNF (LMBP 2529) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Marmenout et al., 1985.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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