Last data update: 28 October 2020 04:17 CET
Plasmid name: pATHTNF (LMBP 2529)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Human tumor necrosis factor cDNA (TNF)
|Selection marker:||Tetracycline (tet)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||This plasmid was constructed as follows: dG/dC-tailed, human TNF cDNA was inserted into the filled-in PstI site of pAT153. The orientation of the inserted fragment was confirmed by an EcoRI digestion.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727524.1.
The nucleotide sequence of the human TNF cDNA corresponds with the EMBL Nucleotide Sequence Database accession number X01394.1.
Name mentioned in Marmenout et al. (1985) is phTNF1.
|EMBL Accession number:||X01394.1, view at EMBL, GenBank, DDBJ
LT727524.1, view at EMBL, GenBank, DDBJ
|Latest sequence update:||04/01/1991|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BamHI, Eco47III, EcoRI, HincII/RsaI and PstI.|
|History of deposit:||This plasmid was deposited by Prof. Dr W. Fiers(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Marmenout et al., Eur. J. Biochem. 152 (1985), 515-522 [PMID: 3932069]
|Related plasmid reference:||Söderberg et al. 43 (2007), 90-99 [PMID: 17561097] [DOI: 10.1016/j.freeradbiomed.2007.03.026]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH1(λ)|
|Host reference:||Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219
|Cultivation medium:||LB-Lennox + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pATHTNF (LMBP 2529) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Marmenout et al., 1985.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.