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GeneCorner plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pATHYG (LMBP 2674)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pathyg.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Herpes simplex virus (HSV) thymidine kinase promoter (TK)
Ribosome
binding site:
-
Terminator: Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)
Selection marker: Ampicillin (amp)
Hygromycin (hyg)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Mammalian cells
Parental clone: pAT153; p220.2
Further information: The plasmid was constructed by cloning the hygromycin B resistance gene (hyg) as a BamHI-BsmI fragment from p220.2 into the BamHI-BsmI opened pAT153 vector.
The bacterial gene for hygromycin B resistance is expressed in mammalian cells using the promoter and polyadenylation signals of the Herpes simplex thymidine kinase gene.
The nucleotide sequence of the hygromycin resistance gene corresponds with the EMBL Nucleotide Sequence database accession number U40398.1.
EMBL Accession number: U40398.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 04/02/1992
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Gritz et al., Gene 25 (1983), 179-188 [PMID: 6319235]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pATHYG (LMBP 2674) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in Gritz et al., 1983.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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