Last data update: 25 February 2021 04:14 CET
Plasmid name: pATHYG (LMBP 2674)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Promoter:||Herpes simplex virus (HSV) thymidine kinase promoter (TK)|
|Terminator:||Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)|
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli
|Parental clone:||pAT153; p220.2|
|Further information:||The plasmid was constructed by cloning the hygromycin B resistance gene (hyg) as a BamHI-BsmI fragment from p220.2 into the BamHI-BsmI opened pAT153 vector.
The bacterial gene for hygromycin B resistance is expressed in mammalian cells using the promoter and polyadenylation signals of the Herpes simplex thymidine kinase gene.
The nucleotide sequence of the hygromycin resistance gene corresponds with the EMBL Nucleotide Sequence database accession number U40398.1.
|EMBL Accession number:||U40398.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||04/02/1992|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Gritz et al., Gene 25 (1983), 179-188 [PMID: 6319235]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pATHYG (LMBP 2674) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in Gritz et al., 1983.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.