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GeneCorner plasmid details

Last data update: 31 October 2020 04:21 CET

Plasmid name: pATIPMA (LMBP 1774)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: patipma.gb (View with Genome Compiler)
patipma.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Influenza A/Puerto Rico/8/34 matrix cDNA (IPM)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pAT153; pIPM
Further information: The plasmid was constructed by inserting the MspI (HpaII) fragment of pIPM, containing cDNA of the A/Puerto Rico/8/34 influenza matrix gene, into the unique ClaI site of pAT153. The inserted fragment is anticlockwise orientated.
The tetracycline resistance is lost due to insertion of the cloned gene in the tetracycline promoter region.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727589.1.
Other names of the plasmid are pATM2 and pATMA.
EMBL Accession number: LT727589.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 06/11/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: NcoI, RsaI and StuI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by A. Raeymaekers(1) and Prof. Dr W. Min Jou(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Neirynck et al., Nat. Med. 5 (1999), 1157-1163 [PMID: 10502819]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pATIPMA (LMBP 1774) is available at BCCM/GeneCorner. This plasmid was deposited by A. Raeymaekers and Prof. Dr W. Min Jou and was published in Neirynck et al., 1999.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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